Department of Neurology
Cell Biology | Molecular and Cellular Neuroscience | Nervous System Diseases | Neurology
Microsatellite expansions are the leading cause of numerous neurodegenerative disorders. Here we demonstrate that GGGGCC and CAG microsatellite repeat RNAs associated with C9orf72 in ALS/FTD and with polyglutamine diseases, respectively, localize to neuritic granules that undergo active transport into distal neuritic segments. In cultured mammalian spinal cord neurons, the presence of neuritic GGGGCC repeat RNA correlates with neuronal branching defects and the repeat RNA localizes to granules that label with FMRP, a transport granule component. Using a Drosophila GGGGCC expansion disease model, we characterize dendritic branching defects that are modulated by FMRP and Orb2. The human orthologues of these modifiers are misregulated in induced pluripotent stem cell-differentiated neurons from GGGGCC expansion carriers. These data suggest that expanded repeat RNAs interact with the mRNA transport and translation machinery, causing transport granule dysfunction. This could be a novel mechanism contributing to the neuronal defects associated with C9orf72 and other microsatellite expansion diseases.
D. melanogaster, cell biology
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© 2015, Schweizer Burguete et al. This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
DOI of Published Version
Elife. 2015 Dec 9;4. pii: e08881. doi: 10.7554/eLife.08881. Link to article on publisher's site
Burguete, Alondra Schweizer; Almeida, Sandra; Gao, Fen-Biao; Kalb, Robert; Akins, Michael R.; and Bonini, Nancy M., "GGGGCC microsatellite RNA is neuritically localized, induces branching defects, and perturbs transport granule function" (2015). Open Access Articles. 2644.
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This work is licensed under a Creative Commons Attribution 4.0 License.