Cellular responses to substrate topography: role of myosin II and focal adhesion kinase

UMMS Affiliation

Department of Physiology

Publication Date


Document Type



3T3 Cells; Animals; Biocompatible Materials; Cell Adhesion; Cell Aggregation; Cell Culture Techniques; Cell Movement; Focal Adhesion Protein-Tyrosine Kinases; Mice; Myosin Type II; Surface Properties


Kinesiology | Physiology


Although two-dimensional cultures have been used extensively in cell biological research, most cells in vivo exist in a three-dimensional environment with complex topographical features, which may account for at least part of the striking differences between cells grown in vivo and in vitro. To investigate how substrate topography affects cell shape and movement, we plated fibroblasts on chemically identical polystyrene substrates with either flat surfaces or micron-sized pillars. Compared to cells on flat surfaces, 3T3 cells on pillar substrates showed a more branched shape, an increased linear speed, and a decreased directional stability. These responses may be attributed to stabilization of cell adhesion on pillars coupled to myosin II-dependent contractions toward pillars. Moreover, using FAK-/- fibroblasts we showed that focal adhesion kinase, or FAK, is essential for the responses to substrate topography. We propose that increased surface contact provided by topographic features guides cell migration by regulating the strength of local adhesions and contractions, through a FAK- and myosin II-dependent mechanism.

DOI of Published Version



Biophys J. 2006 May 15;90(10):3774-82. Epub 2006 Feb 24. Link to article on publisher's site

Journal/Book/Conference Title

Biophysical journal

Related Resources

Link to article in PubMed

PubMed ID