UMMS Affiliation
Department of Medicine, Division of Cardiovascular Medicine; Department of Quantitative Health Sciences
Publication Date
2015-07-06
Document Type
Article
Disciplines
Bacterial Infections and Mycoses | Cardiovascular Diseases | Hemic and Immune Systems
Abstract
INTRODUCTION: Diverse and multi-factorial processes contribute to the progression of cardiovascular disease. These processes affect cells involved in the development of this disease in varying ways, ultimately leading to atherothrombosis. The goal of our study was to compare the differential effects of specific stimuli - two bacterial infections and a Western diet - on platelet responses in ApoE-/- mice, specifically examining inflammatory function and gene expression. Results from murine studies were verified using platelets from participants of the Framingham Heart Study (FHS; n = 1819 participants).
METHODS: Blood and spleen samples were collected at weeks 1 and 9 from ApoE-/- mice infected with Porphyromonas gingivalis or Chlamydia pneumoniae and from mice fed a Western diet for 9 weeks. Transcripts based on data from a Western diet in ApoE-/- mice were measured in platelet samples from FHS using high throughput qRT-PCR.
RESULTS:At week 1, both bacterial infections increased circulating platelet-neutrophil aggregates. At week 9, these cells individually localized to the spleen, while Western diet resulted in increased platelet-neutrophil aggregates in the spleen only. Microarray analysis of platelet RNA from infected or Western diet-fed mice at week 1 and 9 showed differential profiles. Genes, such as Serpina1a, Ttr, Fgg, Rpl21, and Alb, were uniquely affected by infection and diet. Results were reinforced in platelets obtained from participants of the FHS.
CONCLUSION: Using both human studies and animal models, results demonstrate that variable sources of inflammatory stimuli have the ability to influence the platelet phenotype in distinct ways, indicative of the diverse function of platelets in thrombosis, hemostasis, and immunity.
Keywords
Bacterial diseases, Chlamydophila pneumoniae, Diet, Gene expression, Inflammation, Microarrays, Platelets, Spleen
Rights and Permissions
Copyright: © 2015 Beaulieu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
DOI of Published Version
10.1371/journal.pone.0131688
Source
PLoS One. 2015 Jul 6;10(7):e0131688. doi: 10.1371/journal.pone.0131688. eCollection 2015. Link to article on publisher's site
Journal/Book/Conference Title
PloS one
Related Resources
PubMed ID
26148065
Repository Citation
Beaulieu, Lea M.; Clancy, Lauren; Tanriverdi, Kahraman; Benjamin, Emelia J.; Kramer, Carolyn D.; Weinberg, Ellen O.; He, Xianbao; Mekasha, Samrawit; Mick, Eric O.; Ingalls, Robin R.; Genco, Caroline A.; and Freedman, Jane E., "Specific Inflammatory Stimuli Lead to Distinct Platelet Responses in Mice and Humans" (2015). Open Access Articles. 2548.
https://escholarship.umassmed.edu/oapubs/2548
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Included in
Bacterial Infections and Mycoses Commons, Cardiovascular Diseases Commons, Hemic and Immune Systems Commons