Department of Neurology
Cell Biology | Developmental Biology | Genetics and Genomics | Neurology
Intragenic microRNAs (miRNAs), located mostly in the introns of protein-coding genes, are often co-expressed with their host mRNAs. However, their functional interaction in development is largely unknown. Here we show that in Drosophila, miR-92a and miR-92b are embedded in the intron and 3'UTR of jigr1, respectively, and co-expressed with some jigr1 isoforms. miR-92a and miR-92b are highly expressed in neuroblasts of larval brain where Jigr1 expression is low. Genetic deletion of both miR-92a and miR-92b demonstrates an essential cell-autonomous role for these miRNAs in maintaining neuroblast self-renewal through inhibiting premature differentiation. We also show that miR-92a and miR-92b directly target jigr1 in vivo and that some phenotypes due to the absence of these miRNAs are partially rescued by reducing the level of jigr1. These results reveal a novel function of the miR-92 family in Drosophila neuroblasts and provide another example that local negative feedback regulation of host genes by intragenic miRNAs is essential for animal development.
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Copyright: © 2015 Yuva-Aydemir et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI of Published Version
PLoS Genet. 2015 May 22;11(5):e1005264. doi: 10.1371/journal.pgen.1005264. eCollection 2015. Link to article on publisher's site
Yuva-Aydemir, Yeliz; Xu, Xia-Lian; Aydemir, Ozkan; Gascon, Eduardo; Sayin, Serkan; Zhou, Wenke; Hong, Yang; and Gao, Fen-Biao, "Downregulation of the Host Gene jigr1 by miR-92 Is Essential for Neuroblast Self-Renewal in Drosophila" (2015). Open Access Articles. 2532.
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