UMMS Affiliation
Department of Neurobiology; Tzumin Lee Lab; Graduate School of Biomedical Sciences, Neuroscience Program
Publication Date
2012-11-20
Document Type
Article
Subjects
Drosophila; Arthropod Antennae; Neurons; Receptors, Notch
Disciplines
Molecular and Cellular Neuroscience | Neuroscience and Neurobiology
Abstract
Binary cell fate decisions allow the production of distinct sister neurons from an intermediate precursor. Neurons are further diversified based on the birth order of intermediate precursors. Here we examined the interplay between binary cell fate and birth-order-dependent temporal fate in the Drosophila lateral antennal lobe (lAL) neuronal lineage. Single-cell mapping of the lAL lineage by twin-spot mosaic analysis with repressible cell markers (ts-MARCM) revealed that projection neurons (PNs) and local interneurons (LNs) are made in pairs through binary fate decisions. Forty-five types of PNs innervating distinct brain regions arise in a stereotyped sequence; however, the PNs with similar morphologies are not necessarily born in a contiguous window. The LNs are morphologically less diverse than the PNs, and the sequential morphogenetic changes in the two pairs occur independently. Sanpodo-dependent Notch activity promotes and patterns the LN fates. By contrast, Notch diversifies PN temporal fates in a Sanpodo-dispensable manner. These pleiotropic Notch actions underlie the differential temporal fate specification of twin neurons produced by common precursors within a lineage, possibly by modulating postmitotic neurons' responses to Notch-independent transcriptional cascades.
Rights and Permissions
Copyright: © 2012 Lin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI of Published Version
10.1371/journal.pbio.1001425
Source
PLoS Biol. 2012 Nov;10(11):e1001425. doi: 10.1371/journal.pbio.1001425. Link to article on publisher's site
Journal/Book/Conference Title
PLoS biology
Related Resources
PubMed ID
23185131
Repository Citation
Lin S, Kao C, Yu H, Huang Y, Lee T. (2012). Lineage analysis of Drosophila lateral antennal lobe neurons reveals notch-dependent binary temporal fate decisions. Open Access Articles. https://doi.org/10.1371/journal.pbio.1001425. Retrieved from https://escholarship.umassmed.edu/oapubs/2376
Comments
Co-author Suewei Lin is a student in the Neuroscience program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.