UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology
Publication Date
2012-03-15
Document Type
Article
Subjects
Antibiotics, Antineoplastic; Bacterial Proteins; Bleomycin; Culture Media; DNA Breaks, Double-Stranded; DNA Ligases; DNA Restriction Enzymes; DNA, Bacterial; DNA-(Apurinic or Apyrimidinic Site) Lyase; Drug Resistance, Bacterial; Escherichia coli K12; Escherichia coli Proteins; Genes, Bacterial; Mutation; Oligonucleotide Array Sequence Analysis; RNA, Bacterial; Recombinational DNA Repair; SOS Response (Genetics)
Disciplines
Biochemistry, Biophysics, and Structural Biology | Life Sciences | Medicine and Health Sciences
Abstract
Bleomycin (BLM) is a glycopeptide antibiotic and anti-tumor agent that targets primarily the furanose rings of DNA and in the presence of ferrous ions produces oxidative damage and DNA strand breaks. Escherichia coli cells growing in broth medium and exposed to low concentrations of BLM contain double-strand breaks and require homologous recombination to survive. To a lesser extent, the cells also require the abasic (AP) endonucleases associated with base excision repair, presumably to repair oxidative damage. As expected, there is strong induction of the SOS system in treated cells. In contrast, E. coli cells growing in glucose or glycerol minimal medium are resistant to the lethal action of BLM and do not require either homologous recombination functions or AP-endonucleases for survival. DNA ligase activity, however, is needed for cells growing in minimal medium to resist the lethal effects of BLM. There is weak SOS induction in such treated cells.
Rights and Permissions
Copyright: © 2012 Xu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI of Published Version
10.1371/journal.pone.0033256
Source
Xu T, Brown W, Marinus MG (2012) Bleomycin Sensitivity in Escherichia coli is Medium-Dependent. PLoS ONE 7(3): e33256. doi:10.1371/journal.pone.0033256. Link to article on publisher's site
Journal/Book/Conference Title
PloS one
Related Resources
PubMed ID
22438905
Repository Citation
Xu, Tao; Brown, William; and Marinus, Martin G., "Bleomycin sensitivity in Escherichia coli is medium-dependent" (2012). Open Access Articles. 2338.
https://escholarship.umassmed.edu/oapubs/2338
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Medicine and Health Sciences Commons