UMMS Affiliation

Department of Molecular Genetics and Microbiology

Publication Date

9-9-2009

Document Type

Article

Subjects

Anaphase; Cell Cycle Proteins; Cytokinesis; Enzyme Activation; GTP Phosphohydrolases; Gene Knockdown Techniques; Guanine Nucleotide Exchange Factors; Mitotic Spindle Apparatus; Phenotype; Protein-Serine-Threonine Kinases; Recombinant Fusion Proteins; Saccharomyces cerevisiae Proteins; *Schizosaccharomyces; Schizosaccharomyces pombe Proteins; Signal Transduction

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Cytokinesis must be initiated only after chromosomes have been segregated in anaphase and must be terminated once cleavage is completed. We show that the fission yeast protein Etd1 plays a central role in both of these processes. Etd1 activates the guanosine triphosphatase (GTPase) Spg1 to trigger signaling through the septum initiation network (SIN) pathway and onset of cytokinesis. Spg1 is activated in late anaphase when spindle elongation brings spindle pole body (SPB)-localized Spg1 into proximity with its activator Etd1 at cell tips, ensuring that cytokinesis is only initiated when the spindle is fully elongated. Spg1 is active at just one of the two SPBs during cytokinesis. When the actomyosin ring finishes constriction, the SIN triggers disappearance of Etd1 from the half of the cell with active Spg1, which then triggers Spg1 inactivation. Asymmetric activation of Spg1 is crucial for timely inactivation of the SIN. Together, these results suggest a mechanism whereby cell asymmetry is used to monitor cytoplasmic partitioning to turn off cytokinesis signaling.

Rights and Permissions

© 2009 García-Cortés and McCollum This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

DOI of Published Version

10.1083/jcb.200902116

Source

J Cell Biol. 2009 Sep 7;186(5):739-53. Link to article on publisher's site

Journal/Book/Conference Title

The Journal of cell biology

Related Resources

Link to Article in PubMed

PubMed ID

19736319

Creative Commons License

Creative Commons Attribution-Noncommercial-Share Alike 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.

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