The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clusters
Program in Molecular Medicine; Department of Biochemistry and Molecular Pharmacology; Program in Bioinformatics and Integrative Biology
Animals; Chromatin Immunoprecipitation; Chromosomal Proteins, Non-Histone; *DNA Transposable Elements; Drosophila Proteins; Drosophila melanogaster; *Gene Silencing; Heterochromatin; RNA, Small Interfering; Transcription, Genetic
Life Sciences | Medicine and Health Sciences
Piwi-interacting RNAs (piRNAs) silence transposons and maintain genome integrity during germline development. In Drosophila, transposon-rich heterochromatic clusters encode piRNAs either on both genomic strands (dual-strand clusters) or predominantly one genomic strand (uni-strand clusters). Primary piRNAs derived from these clusters are proposed to drive a ping-pong amplification cycle catalyzed by proteins that localize to the perinuclear nuage. We show that the HP1 homolog Rhino is required for nuage organization, transposon silencing, and ping-pong amplification of piRNAs. rhi mutations virtually eliminate piRNAs from the dual-strand clusters and block production of putative precursor RNAs from both strands of the major 42AB dual-strand cluster, but not of transcripts or piRNAs from the uni-strand clusters. Furthermore, Rhino protein associates with the 42AB dual-strand cluster,but does not bind to uni-strand cluster 2 or flamenco. Rhino thus appears to promote transcription of dual-strand clusters, leading to production of piRNAs that drive the ping-pong amplification cycle.
DOI of Published Version
Cell. 2009 Sep 18;138(6):1137-49. Epub 2009 Sep 3. Link to article on publisher's site
Klattenhoff, Carla Andrea; Xi, Hualin; Li, Chengjian; Lee, Soohyun; Xu, Jia; Khurana, Jaspreet S.; Zhang, Fan; Schultz, Nadine; Koppetsch, Birgit S.; Nowosielska, Anetta; Seitz, Herve; Zamore, Phillip D.; Weng, Zhiping; and Theurkauf, William E., "The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clusters" (2009). Open Access Articles. 2106.