The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clusters
Program in Molecular Medicine; Department of Biochemistry and Molecular Pharmacology; Program in Bioinformatics and Integrative Biology
Animals; Chromatin Immunoprecipitation; Chromosomal Proteins, Non-Histone; *DNA Transposable Elements; Drosophila Proteins; Drosophila melanogaster; *Gene Silencing; Heterochromatin; RNA, Small Interfering; Transcription, Genetic
Life Sciences | Medicine and Health Sciences
Piwi-interacting RNAs (piRNAs) silence transposons and maintain genome integrity during germline development. In Drosophila, transposon-rich heterochromatic clusters encode piRNAs either on both genomic strands (dual-strand clusters) or predominantly one genomic strand (uni-strand clusters). Primary piRNAs derived from these clusters are proposed to drive a ping-pong amplification cycle catalyzed by proteins that localize to the perinuclear nuage. We show that the HP1 homolog Rhino is required for nuage organization, transposon silencing, and ping-pong amplification of piRNAs. rhi mutations virtually eliminate piRNAs from the dual-strand clusters and block production of putative precursor RNAs from both strands of the major 42AB dual-strand cluster, but not of transcripts or piRNAs from the uni-strand clusters. Furthermore, Rhino protein associates with the 42AB dual-strand cluster,but does not bind to uni-strand cluster 2 or flamenco. Rhino thus appears to promote transcription of dual-strand clusters, leading to production of piRNAs that drive the ping-pong amplification cycle.
DOI of Published Version
Cell. 2009 Sep 18;138(6):1137-49. Epub 2009 Sep 3. Link to article on publisher's site
Klattenhoff CA, Xi H, Li C, Lee S, Xu J, Khurana JS, Zhang F, Schultz N, Koppetsch BS, Nowosielska A, Seitz H, Zamore PD, Weng Z, Theurkauf WE. (2009). The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clusters. Open Access Publications by UMMS Authors. https://doi.org/10.1016/j.cell.2009.07.014. Retrieved from https://escholarship.umassmed.edu/oapubs/2106