Cross-subtype antibody and cellular immune responses induced by a polyvalent DNA prime-protein boost HIV-1 vaccine in healthy human volunteers
Laboratory of Nucleic Acid Vaccines; Department of Medicine, Division of Infectious Diseases and Immunology
AIDS Vaccines; Adolescent; Adult; HIV Antibodies; HIV Envelope Protein gp120; Human Experimentation; Humans; Immunoglobulin G; Interferon-gamma; Middle Aged; Neutralization Tests; T-Lymphocytes; Vaccines, DNA
Life Sciences | Medicine and Health Sciences
An optimally effective AIDS vaccine would likely require the induction of both neutralizing antibody and cell-mediated immune responses, which has proven difficult to obtain in previous clinical trials. Here we report on the induction of Human Immunodeficiency Virus Type-1 (HIV-1)-specific immune responses in healthy adult volunteers that received the multi-gene, polyvalent, DNA prime-protein boost HIV-1 vaccine formulation, DP6-001, in a Phase I clinical trial conducted in healthy adult volunteers of both genders. Robust cross-subtype HIV-1-specific T cell responses were detected in IFNgamma ELISPOT assays. Furthermore, we detected high titer serum antibody responses that recognized a wide range of primary HIV-1 Env antigens and also neutralized pseudotyped viruses that express the primary Env antigens from multiple HIV-1 subtypes. These findings demonstrate that the DNA prime-protein boost approach is an effective immunization method to elicit both humoral and cell-mediated immune responses in humans, and that a polyvalent Env formulation could generate broad immune responses against HIV-1 viruses with diverse genetic backgrounds.
DOI of Published Version
Vaccine. 2008 Feb 20;26(8):1098-110. Epub 2008 Jan 10. Link to article on publisher's site
Wang S, Kennedy JS, West K, Montefiori DC, Coley SE, Lawrence JM, Shen S, Green S, Rothman AL, Ennis FA, Arthos J, Pal R, Markham P, Lu S. (2008). Cross-subtype antibody and cellular immune responses induced by a polyvalent DNA prime-protein boost HIV-1 vaccine in healthy human volunteers. Open Access Publications by UMMS Authors. https://doi.org/10.1016/j.vaccine.2007.12.024. Retrieved from https://escholarship.umassmed.edu/oapubs/2057