Linking SNPs to CAG repeat length in Huntington's disease patients
Authors
Liu, WanzhaoKennington, Lori A.
Rosas, H. Diana
Hersch, Steven M.
Cha, Jang-Ho
Zamore, Phillip D.
Aronin, Neil
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyDepartment of Medicine, Division of Endocrinology and Metabolism
Document Type
Journal ArticlePublication Date
2008-10-22Keywords
HumansHuntington Disease
Molecular Sequence Data
Polymorphism, Single Nucleotide
Trinucleotide Repeats
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Allele-specific silencing using small interfering RNAs targeting heterozygous single-nucleotide polymorphisms (SNPs) is a promising therapy for human trinucleotide repeat diseases such as Huntington's disease. Linking SNP identities to the two HTT alleles, normal and disease-causing, is a prerequisite for allele-specific RNA interference. Here we describe a method, SNP linkage by circularization (SLiC), to identify linkage between CAG repeat length and nucleotide identity of heterozygous SNPs using Huntington's disease patient peripheral blood samples.Source
Nat Methods. 2008 Nov;5(11):951-3. Epub 2008 Oct 19. Link to article on publisher's site
DOI
10.1038/nmeth.1261Permanent Link to this Item
http://hdl.handle.net/20.500.14038/39166PubMed ID
18931668Related Resources
ae974a485f413a2113503eed53cd6c53
10.1038/nmeth.1261