PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans
Program in Molecular Medicine
Animals; Base Sequence; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Fertility; Gene Expression Regulation; Germ Cells; Models, Biological; Molecular Sequence Data; Mutation; Protein Binding; RNA, Helminth; RNA, Small Interfering; RNA-Induced Silencing Complex; Regulatory Sequences, Nucleic Acid
Life Sciences | Medicine and Health Sciences
In metazoans, Piwi-related Argonaute proteins have been linked to germline maintenance, and to a class of germline-enriched small RNAs termed piRNAs. Here we show that an abundant class of 21 nucleotide small RNAs (21U-RNAs) are expressed in the C. elegans germline, interact with the C. elegans Piwi family member PRG-1, and depend on PRG-1 activity for their accumulation. The PRG-1 protein is expressed throughout development and localizes to nuage-like structures called P granules. Although 21U-RNA loci share a conserved upstream sequence motif, the mature 21U-RNAs are not conserved and, with few exceptions, fail to exhibit complementarity or evidence for direct regulation of other expressed sequences. Our findings demonstrate that 21U-RNAs are the piRNAs of C. elegans and link this class of small RNAs and their associated Piwi Argonaute to the maintenance of temperature-dependent fertility.
DOI of Published Version
Mol Cell. 2008 Jul 11;31(1):67-78. Epub 2008 Jun 19. Link to article on publisher's site
Batista PJ, Ruby JG, Claycomb JM, Chiang HR, Fahlgren N, Kasschau KD, Chaves DA, Gu W, Vasale JJ, Duan S, Conte D, Luo S, Schroth GP, Carrington JC, Bartel DP, Mello CC. (2008). PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans. Open Access Publications by UMMS Authors. https://doi.org/10.1016/j.molcel.2008.06.002. Retrieved from https://escholarship.umassmed.edu/oapubs/1908