Transcription of histone H4, H3, and H1 cell cycle genes: promoter factor HiNF-D contains CDC2, cyclin A, and an RB-related protein
Department of Cell Biology
Alkaloids; Base Sequence; CDC2 Protein Kinase; *Cell Cycle; Cyclins; DNA-Binding Proteins; Ethers, Cyclic; Gene Expression Regulation; Histones; Humans; Macromolecular Substances; Molecular Sequence Data; Okadaic Acid; Oligodeoxyribonucleotides; Phosphorylation; *Promoter Regions (Genetics); RNA, Messenger; Retinoblastoma Protein; Staurosporine; Trans-Activation (Genetics); Transcription Factors; *Transcription, Genetic
Life Sciences | Medicine and Health Sciences
Cell cycle-controlled human histone genes are coordinately expressed during S phase, and transcriptional regulation involves a series of trans-acting factors (HiNFs). The proliferation-specific factor HiNF-D interacts with multiple recognition motifs in histone H4, H3, and H1 promoters. Using gel shift immunoassays, we show that CDC2, cyclin A, and an RB-related protein are ubiquitous subunits of HiNF-D binding activity isolated from several cell types. HiNF-D levels in vivo are sensitive to okadaic acid and staurosporine, indicating that HiNF-D activity and/or assembly is influenced by phosphorylation status. Thus, HiNF-D appears to be a multicomponent phosphoprotein that participates in coordinate control of multiple histone H4, H3, and H1 genes during the cell cycle. The presence of cell cycle mediators in the HiNF-D complex suggests linkage between transcriptional control of histones, enzymes involved in DNA synthesis, and the onset of DNA replication during the G1/S phase transition.
Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12882-6.
Proceedings of the National Academy of Sciences of the United States of America
Van Wijnen AJ, Aziz F, Grana X, De Luca A, Desai RK, Jaarsveld K, Last TJ, Soprano KJ, Giordano A, Lian JB. (1994). Transcription of histone H4, H3, and H1 cell cycle genes: promoter factor HiNF-D contains CDC2, cyclin A, and an RB-related protein. Open Access Publications by UMMS Authors. Retrieved from https://escholarship.umassmed.edu/oapubs/1806