Transcription of histone H4, H3, and H1 cell cycle genes: promoter factor HiNF-D contains CDC2, cyclin A, and an RB-related protein

UMMS Affiliation

Department of Cell Biology

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Document Type



Alkaloids; Base Sequence; CDC2 Protein Kinase; *Cell Cycle; Cyclins; DNA-Binding Proteins; Ethers, Cyclic; Gene Expression Regulation; Histones; Humans; Macromolecular Substances; Molecular Sequence Data; Okadaic Acid; Oligodeoxyribonucleotides; Phosphorylation; *Promoter Regions (Genetics); RNA, Messenger; Retinoblastoma Protein; Staurosporine; Trans-Activation (Genetics); Transcription Factors; *Transcription, Genetic


Life Sciences | Medicine and Health Sciences


Cell cycle-controlled human histone genes are coordinately expressed during S phase, and transcriptional regulation involves a series of trans-acting factors (HiNFs). The proliferation-specific factor HiNF-D interacts with multiple recognition motifs in histone H4, H3, and H1 promoters. Using gel shift immunoassays, we show that CDC2, cyclin A, and an RB-related protein are ubiquitous subunits of HiNF-D binding activity isolated from several cell types. HiNF-D levels in vivo are sensitive to okadaic acid and staurosporine, indicating that HiNF-D activity and/or assembly is influenced by phosphorylation status. Thus, HiNF-D appears to be a multicomponent phosphoprotein that participates in coordinate control of multiple histone H4, H3, and H1 genes during the cell cycle. The presence of cell cycle mediators in the HiNF-D complex suggests linkage between transcriptional control of histones, enzymes involved in DNA synthesis, and the onset of DNA replication during the G1/S phase transition.


Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12882-6.

Journal/Book/Conference Title

Proceedings of the National Academy of Sciences of the United States of America

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