UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyDocument Type
Journal ArticlePublication Date
1988-10-25Keywords
*Base SequenceColiphages
*DNA Mutational Analysis
Methylnitronitrosoguanidine
*Mutation
*Streptozocin
Tetracycline Resistance
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
The isolation and characterization of streptozotocin (STZ)-induced mutations in the phage P22 mnt repressor gene is described. Cells carrying the plasmid-borne mnt gene were exposed to STZ to give 10-20 percent survival and at least an eleven-fold increase in mutation frequency. DNA sequence analysis showed that 50 of 51 STZ-induced mutations were GC to AT transitions, and one was an AT to GC transition. We have also compared the STZ mutational spectrum to that for N-methyl-N'-nitro-N-nitroso-guanidine (MNNG). There are sites in the mnt gene which are mutated only by STZ; only by MNNG, or by both agents. Sites at which only STZ induced GC to AT transition mutations occur were in sequences that are pyrimidine rich 5' to the mutated site and purine rich 3' to the mutated site. Induction of mutations by both STZ and MNNG should be considered to maximize the number of mutable sites.Source
Nucleic Acids Res. 1988 Oct 25;16(20):9811-20. Link to article on publisher's websiteDOI
10.1093/nar/16.20.9811Permanent Link to this Item
http://hdl.handle.net/20.500.14038/38877PubMed ID
2972994Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1093/nar/16.20.9811