Program in Molecular Medicine
Activating Transcription Factors; Animals; Binding Sites; Blood Proteins; Cell Line; Cyclic AMP Response Element-Binding; Protein; DNA; Fungal Proteins; Gene Expression; Haplorhini; Macromolecular Substances; Plasmids; Protein Kinases; Recombinant Fusion Proteins; *Saccharomyces cerevisiae Proteins; Transcription Factors; Transcription, Genetic
Life Sciences | Medicine and Health Sciences
Cyclic AMP response element binding protein (CREB) activates transcription of cAMP response element (CRE)-containing promoters following an elevation of intracellular cAMP. Here we show that CREB and the highly related protein ATF-1 are also potent transcription inhibitors. Strikingly, CREB inhibits transcription of multiple activators, whose DNA-binding domains and activation regions are unrelated to one another. Inhibition requires that the CREB dimerization and DNA-binding domains are intact. However, inhibition is not dependent upon the presence of a CRE in the promoter, and does not involve heterodimer formation between CREB and the activator. The ability of an activator protein to inhibit transcription in such a promiscuous fashion has not been previously reported.
Nucleic Acids Res. 1993 Jun 25;21(12):2907-11.
Nucleic acids research
Lemaigre FP, Ace CI, Green MR. (1993). The cAMP response element binding protein, CREB, is a potent inhibitor of diverse transcriptional activators. Open Access Publications by UMass Chan Authors. Retrieved from https://escholarship.umassmed.edu/oapubs/1708