Title
Controlling gene expression in living cells through small molecule-RNA interactions
UMMS Affiliation
Program in Molecular Medicine
Publication Date
1998-10-09
Document Type
Article
Subjects
Animals; Anti-Bacterial Agents; Base Sequence; Benzimidazoles; Bisbenzimide; CHO Cells; Cricetinae; Drug Resistance, Microbial; Escherichia coli; *Gene Expression Regulation; Kanamycin; Ligands; Molecular Sequence Data; Protein Biosynthesis; RNA; RNA, Messenger; Tobramycin; Transfection
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Short RNA aptamers that specifically bind to a wide variety of ligands in vitro can be isolated from randomized pools of RNA. Here it is shown that small molecule aptamers also bound their ligand in vivo, enabling development of a method for controlling gene expression in living cells. Insertion of a small molecule aptamer into the 5' untranslated region of a messenger RNA allowed its translation to be repressible by ligand addition in vitro as well as in mammalian cells. The ability of small molecules to control expression of specific genes could facilitate studies in many areas of biology and medicine.
DOI of Published Version
10.1126/science.282.5387.296
Source
Science. 1998 Oct 9;282(5387):296-8.
Journal/Book/Conference Title
Science (New York, N.Y.)
Related Resources
PubMed ID
9765156
Repository Citation
Werstuck, Geoffrey and Green, Michael R., "Controlling gene expression in living cells through small molecule-RNA interactions" (1998). Open Access Articles. 1643.
https://escholarship.umassmed.edu/oapubs/1643