Title
Requirement of JNK for stress-induced activation of the cytochrome c-mediated death pathway
UMMS Affiliation
Howard Hughes Medical Institute and Program in Molecular Medicine; Department of Biochemistry and Molecular Pharmacology; Department of Cell Biology
Publication Date
2000-05-08
Document Type
Article
Subjects
Animals; *Apoptosis; Apoptotic Protease-Activating Factor 1; Caspase 3; Caspase 9; Caspases; Cell Count; Cell Division; Cells, Cultured; Cytochrome c Group; DNA Fragmentation; Enzyme Activation; Fibroblasts; Gene Targeting; JNK Mitogen-Activated Protein Kinases; MAP Kinase Signaling System; Methyl Methanesulfonate; Mice; Mitochondria; Mitogen-Activated Protein Kinases; NF-kappa B; *Protein-Serine-Threonine Kinases; Proteins; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-akt; Proto-Oncogene Proteins c-bcl-2; Tumor Suppressor Protein p53; Ultraviolet Rays
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The c-Jun NH2-terminal kinase (JNK) is activated when cells are exposed to ultraviolet (UV) radiation. However, the functional consequence of JNK activation in UV-irradiated cells has not been established. It is shown here that JNK is required for UV-induced apoptosis in primary murine embryonic fibroblasts. Fibroblasts with simultaneous targeted disruptions of all the functional Jnk genes were protected against UV-stimulated apoptosis. The absence of JNK caused a defect in the mitochondrial death signaling pathway, including the failure to release cytochrome c. These data indicate that mitochondria are influenced by proapoptotic signal transduction through the JNK pathway.
DOI of Published Version
10.1126/science.288.5467.870
Source
Science. 2000 May 5;288(5467):870-4.
Journal/Book/Conference Title
Science (New York, N.Y.)
Related Resources
PubMed ID
10797012
Repository Citation
Tournier C, Hess PM, Yang DD, Xu J, Turner TK, Nimnual AS, Bar-Sagi D, Jones SN, Flavell RA, Davis RJ. (2000). Requirement of JNK for stress-induced activation of the cytochrome c-mediated death pathway. Open Access Publications by UMass Chan Authors. https://doi.org/10.1126/science.288.5467.870. Retrieved from https://escholarship.umassmed.edu/oapubs/1641