Distinct classes of yeast promoters revealed by differential TAF recruitment

UMMS Affiliation

Howard Hughes Medical Institute, Program in Molecular Medicine

Publication Date


Document Type



Cross-Linking Reagents; DNA; DNA-Binding Proteins; Formaldehyde; Mutation; Precipitin Tests; *Promoter Regions (Genetics); Saccharomyces cerevisiae; TATA Box; TATA-Box Binding Protein; Trans-Activators; Transcription Factors; Transcription Factors, TFII


Life Sciences | Medicine and Health Sciences


The transcription factor TFIID contains the TATA box binding protein (TBP) and multiple TBP-associated factors (TAFs). Here, the association of TFIID components with promoters that either are dependent on multiple TAFs (TAFdep) or have no apparent TAF requirement (TAFind) is analyzed in yeast. At TAFdep promoters, TAFs are present at levels comparable to that of TBP, whereas at TAFind promoters, TAFs are present at levels that approximate background. After inactivation of several general transcription factors, including TBP, TAFs are still recruited by activators to TAFdep promoters. The results reveal two classes of promoters: at TAFind promoters, TBP is recruited in the apparent absence of TAFs, whereas at TAFdep promoters, TAFs are co-recruited with TBP in a manner consistent with direct activator-TAF interactions.


Science. 2000 May 19;288(5469):1242-4.

Journal/Book/Conference Title

Science (New York, N.Y.)

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Link to Article in PubMed

PubMed ID