UMMS Affiliation

Department of Pharmacology; Department of Pathology; Department of Molecular Genetics and Microbiology

Publication Date

1987-11-01

Document Type

Article

Subjects

Animals; Aotus trivirgatus; Callitrichinae; Cell Line; *Cell Transformation, Viral; Culture Techniques; DNA Replication; Herpesvirus 2, Saimiriine; Nucleic Acid Hybridization; Plasmids; Species Specificity

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Sequences within the rightmost 7 kilobases of the unique L DNA of herpesvirus saimiri are required for oncogenicity of the virus. The same DNA region has been found to be highly variable among different strains of herpesvirus saimiri. On the basis of this variability, herpesvirus saimiri strains were classified into groups A, B, and non-A, non-B. Herpesvirus saimiri strains representing the three groups were used successfully for in vitro immortalization of phytohemagglutinin-activated, interleukin 2 (IL-2)-expanded peripheral blood lymphocytes of common marmosets (Callithrix jacchus). Peripheral blood leukocytes could be immortalized from only a subset of common marmosets (5 of 13). All of the immortalized cell lines contained covalently closed circular viral DNA molecules and initially showed a low level of virus production. Cells immortalized by group A and group non-A, non-B strains did not require IL-2 in the medium. However, the only group B immortalized cell line, 473-SMHI, did not grow well in the absence of IL-2. The different characteristics of cell lines immortalized by herpesvirus saimiri strains belonging to different groups may help to elucidate some functions coded by the highly variable DNA region which is involved in the oncogenic process.

Source

J Virol. 1987 Nov;61(11):3485-90.

Journal/Book/Conference Title

Journal of virology

Related Resources

Link to Article in PubMed

PubMed ID

2822956

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