Department of Medicine; Center for Infectious Disease and Vaccine Research
Antibodies, Monoclonal; Antibodies, Viral; Antigens, Differentiation; Cell Line; Dengue Virus; Humans; Immunoglobulin G; Interferon-gamma, Recombinant; Monocytes; Receptors, Fc; Receptors, IgG; gamma-Globulins
Life Sciences | Medicine and Health Sciences
It has been reported that anti-dengue antibodies at subneutralizing concentrations augment dengue virus infection of monocytic cells. This is due to the increased uptake of dengue virus in the form of virus-antibody complexes by cells via Fc gamma receptors. We analyzed the effects of recombinant human gamma interferon (rIFN-gamma) on dengue virus infection of human monocytic cells. U937 cells, a human monocytic cell line, were infected with dengue virus in the form of virus-antibody complexes after rIFN-gamma treatment. Pretreatment of U937 cells with rIFN-gamma resulted in a significant increase in the number of dengue virus-infected cells and in the yield of infectious virus. rIFN-gamma did not augment dengue virus infection when cells were infected with virus in the absence of anti-dengue antibodies. Gamma interferon (IFN-gamma) produced by peripheral blood lymphocytes from dengue-immune donors after in vitro stimulation with dengue antigens also augmented dengue virus infection of U937 cells. IFN-gamma did not augment dengue virus infections when cells were infected with virus in the presence of F(ab')2 prepared from anti-dengue immunoglobulin G. Human immunoglobulin inhibited IFN-gamma-induced augmentation. IFN-gamma increased the number of Fc gamma receptors on U937 cells. The increase in the percentage of dengue antigen-positive cells correlated with the increase in the number of Fc gamma receptors after rIFN-gamma treatment. These results indicate that IFN-gamma-induced augmentation of dengue virus infection is Fc gamma receptor mediated. Based on these results we conclude that IFN-gamma increases the number of Fc gamma receptors and that this leads to an augmented uptake of dengue virus in the form of dengue virus-antibody complexes, which results in augmented dengue virus infection.
J Virol. 1988 Nov;62(11):3928-33.
Journal of virology
Kontny U, Kurane I, Ennis FA. (1988). Gamma interferon augments Fc gamma receptor-mediated dengue virus infection of human monocytic cells. Open Access Publications by UMMS Authors. Retrieved from https://escholarship.umassmed.edu/oapubs/1587