UMMS Affiliation

Department of Medicine, Division of Infectious Diseases; Center for Infectious Disease and Vaccine Research

Publication Date

1989-12-01

Document Type

Article

Subjects

Antibodies, Monoclonal; Antigens, CD; Antigens, CD8; Antigens, Differentiation, T-Lymphocyte; Antigens, Viral; Cell Line; Complement System Proteins; Cross Reactions; Cytotoxicity, Immunologic; Dengue Virus; Humans; Lymphocyte Activation; Skin; T-Lymphocytes, Cytotoxic; Ultraviolet Rays

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Stimulation with live dengue virus of peripheral blood mononuclear cells from a dengue virus type 4-immune donor generated virus-specific, serotype-cross-reactive, CD8+, class I-restricted cytotoxic T lymphocytes (CTL) capable of lysing dengue virus-infected cells and cells pulsed with dengue virus antigens of all four serotypes. These CTL lysed autologous fibroblasts infected with vaccinia virus-dengue virus recombinant viruses containing the E gene or several nonstructural dengue virus type 4 genes. These results demonstrate that both dengue virus structural and nonstructural proteins are targets for the cytotoxic T-cell-mediated immune response to dengue virus and suggest that serotype-cross-reactive CD8+ CTL may be important mediators of viral clearance and of virus-induced immunopathology during secondary dengue virus infections.

Source

J Virol. 1989 Dec;63(12):5086-91.

Journal/Book/Conference Title

Journal of virology

Related Resources

Link to Article in PubMed

PubMed ID

2511337

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