Department of Medicine, Division of Infectious Diseases and Immunology; Center for Infectious Disease and Vaccine Research
Amino Acid Sequence; Antibodies, Monoclonal; Antigens, CD8; Antigens, Differentiation, T-Lymphocyte; Cell Line; Clone Cells; Cytotoxicity, Immunologic; Epitopes; Gene Products, gag; HIV Core Protein p24; HIV-1; HLA-C Antigens; Humans; Molecular Sequence Data; Restriction Mapping; T-Lymphocytes, Cytotoxic; Viral Core Proteins
Life Sciences | Medicine and Health Sciences
A unique epitope on the gag protein of human immunodeficiency virus type 1 (HIV-1), located at amino acid 145 to 150, has been mapped by using a CD8+ cytotoxic T-lymphocyte (CTL) clone. This epitope is highly conserved among 18 HIV-1 strains. The HIV-1 gag-specific human leukocyte antigen (HLA) class I-restricted CD8+ CTL clone was generated from fresh peripheral blood mononuclear cells of an HIV-seropositive donor by stimulation with gamma-irradiated allogeneic peripheral blood mononuclear cells in the presence of an anti-CD3 monoclonal antibody and recombinant interleukin-2. This gag-specific CTL clone killed autologous target cells infected with a recombinant vaccinia virus containing the gag gene of HIV-1 and target cells pulsed with an authentic p24gag construct expressed in Escherichia coli. Fine specificity was determined by using a panel of overlapping 30-amino-acid-long synthetic peptides and subsequently using smaller peptides to precisely map the CTL domain on p24. The epitope is on a highly conserved region, and it overlaps with a major B-cell epitope of gag. This CD8+ T-cell epitope is restricted by HLA-Cw3, which has not been previously identified as a restricting element for human CTL responses.
J Virol. 1991 Aug;65(8):4051-6.
Journal of virology
Littaua, Rebecca A.; Oldstone, Michael B. A.; Takeda, Akira; Debouck, Christine; Wong, Johnson T.; Tuazon, Carmelita U.; Moss, Bernard; Kievits, Femia; and Ennis, Francis A., "An HLA-C-restricted CD8+ cytotoxic T-lymphocyte clone recognizes a highly conserved epitope on human immunodeficiency virus type 1 gag" (1991). Open Access Articles. 1571.