UMMS Affiliation

Program in Molecular Medicine; Department of Pediatrics

Publication Date


Document Type



Animals; Base Sequence; Cell Line; Chloramphenicol O-Acetyltransferase; DNA-Binding Proteins; Erythroid-Specific DNA-Binding Factors; Gene Expression Regulation, Viral; HIV Core Protein p24; HIV Long Terminal Repeat; HIV-1; Humans; In Situ Hybridization; Lymphoid Tissue; Molecular Sequence Data; Protein Binding; RNA, Messenger; RNA, Viral; Recombinant Fusion Proteins; Transcription Factors; Transcription, Genetic; Virion; YY1 Transcription Factor


Life Sciences | Medicine and Health Sciences


The transcriptional activity of human immunodeficiency virus type 1 (HIV-1) is affected by many cellular factors. Homologies near the HIV-1 initiator region to the DNA-binding sequences of YY1, a multifunctional transcription factor known to regulate diverse viral and cellular promoters, suggested that YY1 might regulate HIV-1. Antibody to YY1 blocked the formation of complexes by HeLa cell nuclear extract and a DNA oligonucleotide encoding the HIV-1 initiator region. HIV-1 long terminal repeat (LTR) expression, as measured the expression of a transfected LTR-CAT reporter gene, was repressed more than 12-fold by the cotransfection of a YY1 expression vector. HIV-1 production by both COS-1 and CEM cells after transfection of an infectious molecular HIV-1 clone was repressed 7- to 20-fold by cotransfection of a YY1 expression vector. HIV-1 production was also decreased threefold in a CD4-positive lymphocyte cell line chronically infected with HIV-1 (8E5) after transfection of YY1. In situ hybridization studies confirmed that YY1 reduced HIV-1 RNA expression. YY1 may play an important role in the regulation of HIV-1 LTR expression in vivo and virus production by infected cells.


J Virol. 1994 Feb;68(2):905-10.

Journal/Book/Conference Title

Journal of virology

Related Resources

Link to Article in PubMed

PubMed ID




To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.