Laboratory of Nucleic Acid Vaccines; Department of Medicine; Department of Biochemistry and Molecular Pharmacology
Animals; Antibodies, Viral; Cell Line; Cercopithecus aethiops; Cytopathogenic Effect, Viral; Epitopes; Humans; Immunization; Membrane Glycoproteins; Neutralization Tests; Rabbits; SARS Virus; Severe Acute Respiratory Syndrome; Vaccines, DNA; Vero Cells; Viral Envelope Proteins; Viral Vaccines
Life Sciences | Medicine and Health Sciences
The Spike (S) protein of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) plays important roles in viral pathogenesis and potentially in the development of an effective vaccine against this virulent infectious disease. In this study, the codon-optimized S gene of SARS-CoV was synthesized to construct DNA vaccine plasmids expressing either the full-length or segments of the S protein. High titer S-specific immunoglobulin G antibody responses were elicited in rabbits immunized with DNA against various segments of the S protein. Two neutralizing domains were identified on the S protein, one at the N terminus (Ser12-Thr535) and the other near the C terminus (Arg797-Ile1192).
DOI of Published Version
J Virol. 2005 Feb;79(3):1906-10. Link to article on publisher's site
Journal of virology
Wang S, Chou TW, Sakhatskyy PV, Huang S, Lawrence JM, Cao H, Huang X, Lu S. (2005). Identification of two neutralizing regions on the severe acute respiratory syndrome coronavirus spike glycoprotein produced from the mammalian expression system. Open Access Publications by UMMS Authors. https://doi.org/10.1128/JVI.79.3.1906-1910.2005. Retrieved from https://escholarship.umassmed.edu/oapubs/1520