UMMS Affiliation

Laboratory of Nucleic Acid Vaccines; Department of Medicine; Department of Biochemistry and Molecular Pharmacology

Publication Date

2005-01-15

Document Type

Article

Subjects

Animals; Antibodies, Viral; Cell Line; Cercopithecus aethiops; Cytopathogenic Effect, Viral; Epitopes; Humans; Immunization; Membrane Glycoproteins; Neutralization Tests; Rabbits; SARS Virus; Severe Acute Respiratory Syndrome; Vaccines, DNA; Vero Cells; Viral Envelope Proteins; Viral Vaccines

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The Spike (S) protein of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) plays important roles in viral pathogenesis and potentially in the development of an effective vaccine against this virulent infectious disease. In this study, the codon-optimized S gene of SARS-CoV was synthesized to construct DNA vaccine plasmids expressing either the full-length or segments of the S protein. High titer S-specific immunoglobulin G antibody responses were elicited in rabbits immunized with DNA against various segments of the S protein. Two neutralizing domains were identified on the S protein, one at the N terminus (Ser12-Thr535) and the other near the C terminus (Arg797-Ile1192).

DOI of Published Version

10.1128/JVI.79.3.1906-1910.2005

Source

J Virol. 2005 Feb;79(3):1906-10. Link to article on publisher's site

Journal/Book/Conference Title

Journal of virology

Related Resources

Link to Article in PubMed

PubMed ID

15650214

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