Laboratory of Nucleic Acid Vaccines; Department of Medicine; Department of Biochemistry and Molecular Pharmacology
Animals; Antibodies, Viral; Cell Line; Cercopithecus aethiops; Cytopathogenic Effect, Viral; Epitopes; Humans; Immunization; Membrane Glycoproteins; Neutralization Tests; Rabbits; SARS Virus; Severe Acute Respiratory Syndrome; Vaccines, DNA; Vero Cells; Viral Envelope Proteins; Viral Vaccines
Life Sciences | Medicine and Health Sciences
The Spike (S) protein of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) plays important roles in viral pathogenesis and potentially in the development of an effective vaccine against this virulent infectious disease. In this study, the codon-optimized S gene of SARS-CoV was synthesized to construct DNA vaccine plasmids expressing either the full-length or segments of the S protein. High titer S-specific immunoglobulin G antibody responses were elicited in rabbits immunized with DNA against various segments of the S protein. Two neutralizing domains were identified on the S protein, one at the N terminus (Ser12-Thr535) and the other near the C terminus (Arg797-Ile1192).
DOI of Published Version
J Virol. 2005 Feb;79(3):1906-10. Link to article on publisher's site
Journal of virology
Wang, Shixia; Chou, Te-hui W.; Sakhatskyy, Pavlo V.; Huang, Song; Lawrence, John M.; Cao, Hong; Huang, Xiaoyun; and Lu, Shan, "Identification of two neutralizing regions on the severe acute respiratory syndrome coronavirus spike glycoprotein produced from the mammalian expression system" (2005). Open Access Articles. 1520.