Program in Molecular Medicine
Cells, Cultured; Gene Expression Regulation, Viral; HIV Infections; HIV-1; Hela Cells; Humans; Karyopherins; Macrophages; Simian Acquired Immunodeficiency Syndrome; Simian immunodeficiency virus
Life Sciences | Medicine and Health Sciences
In an in vitro assay employing reconstituted nuclei, importin 7 (IPO7) has been implicated in nuclear translocation of human immunodeficiency virus type 1 (HIV-1) cDNA. Using RNA interference technology, we inhibited expression of IPO7 by 80 to 95% in primary macrophages and in HeLa cells and monitored their ability to support HIV-1 and simian immunodeficiency virus (SIV) cDNA synthesis, nuclear translocation, and infection efficiency. Marked IPO7 deficiency did not alter the rate or extent of HIV-1 or SIV cDNA synthesis or nuclear translocation. The infection efficiency of HIV-1 was similarly unaltered. Therefore, in natural, nondividing targets of HIV-1, IPO7 may be dispensable for infection.
DOI of Published Version
J Virol. 2005 Sep;79(17):11541-6. Link to article on publisher's site
Journal of virology
Zielske, Steven P. and Stevenson, Mario, "Importin 7 may be dispensable for human immunodeficiency virus type 1 and simian immunodeficiency virus infection of primary macrophages" (2005). Open Access Articles. 1517.