Program in Molecular Medicine
Cells, Cultured; Gene Expression Regulation, Viral; HIV Infections; HIV-1; Hela Cells; Humans; Karyopherins; Macrophages; Simian Acquired Immunodeficiency Syndrome; Simian immunodeficiency virus
Life Sciences | Medicine and Health Sciences
In an in vitro assay employing reconstituted nuclei, importin 7 (IPO7) has been implicated in nuclear translocation of human immunodeficiency virus type 1 (HIV-1) cDNA. Using RNA interference technology, we inhibited expression of IPO7 by 80 to 95% in primary macrophages and in HeLa cells and monitored their ability to support HIV-1 and simian immunodeficiency virus (SIV) cDNA synthesis, nuclear translocation, and infection efficiency. Marked IPO7 deficiency did not alter the rate or extent of HIV-1 or SIV cDNA synthesis or nuclear translocation. The infection efficiency of HIV-1 was similarly unaltered. Therefore, in natural, nondividing targets of HIV-1, IPO7 may be dispensable for infection.
DOI of Published Version
J Virol. 2005 Sep;79(17):11541-6. Link to article on publisher's site
Journal of virology
Zielske SP, Stevenson M. (2005). Importin 7 may be dispensable for human immunodeficiency virus type 1 and simian immunodeficiency virus infection of primary macrophages. Open Access Articles. https://doi.org/10.1128/JVI.79.17.11541-11546.2005. Retrieved from https://escholarship.umassmed.edu/oapubs/1517