Suppression of insulin receptor substrate 1 (IRS-1) promotes mammary tumor metastasis
Department of Cancer Biology
Animals; Breast Neoplasms; Cell Line, Tumor; Female; Humans; Lung Neoplasms; Male; Mammary Neoplasms, Animal; Mice; Mice, Inbred C57BL; Mice, Knockout; Phosphoproteins
Life Sciences | Medicine and Health Sciences
The insulin receptor substrate (IRS) proteins are cytoplasmic adaptors that organize signaling complexes downstream of activated cell surface receptors. Here, we show that IRS-1 and IRS-2, despite significant homology, play critical yet distinct functions in breast cancer, and we identify specific signaling pathways that are influenced by IRS-1 using the polyoma virus middle-T (PyV-MT) transgenic mouse model of mammary carcinoma and Irs-1 null (Irs1(-/-)) mice. The absence of Irs-1 expression enhanced metastatic spread significantly without a significant effect on primary tumor growth. Orthotopic transplant studies revealed that the increased metastatic potential of Irs1-deficient tumor cells is cell autonomous. Mammary tumors that developed in PyV-MT::Irs1(-/-) mice exhibited elevated Irs-2 function and enhanced phosphatidylinositol 3-kinase/Akt/mTor activity, suggesting that one mechanism by which Irs-1 impedes metastasis is to suppress Irs-2-dependent signaling. In support of this mechanism, reduction of Irs-2 expression in Irs1(-/-) tumor cells restored mTor signaling to wild-type levels. PyV-MT::Irs1(-/-) tumors also exhibited a significant increase in vascular endothelial growth factor expression and microvessel density, which could facilitate their dissemination. The significance of our findings for human breast cancer is heightened by our observation that Irs-1 is inactivated in wild-type, metastatic mammary tumors by serine phosphorylation. Collectively, our findings reveal that inactivation of IRS-1 enhances breast cancer metastasis and support the novel hypothesis that IRS-1 has metastasis suppressor functions for breast cancer.
DOI of Published Version
Mol Cell Biol. 2006 Dec;26(24):9338-51. Epub 2006 Oct 9. Link to article on publisher's site
Molecular and cellular biology
Ma, Zhefu; Gibson, Shannon L.; Byrne, Maura A.; Zhang, Junran; White, Morris F.; and Shaw, Leslie M., "Suppression of insulin receptor substrate 1 (IRS-1) promotes mammary tumor metastasis" (2006). Open Access Articles. 1407.