UMMS Affiliation
Program in Molecular Medicine
Publication Date
1998-10-08
Document Type
Article
Subjects
Amino Acid Sequence; Animals; Carrier Proteins; Cell Division; Cell Nucleolus; Cell Nucleus; Cloning, Molecular; Consensus Sequence; Conserved Sequence; Epidermal Growth Factor; Gene Library; Genetic Complementation Test; Hela Cells; Humans; Mice; Molecular Sequence Data; Saccharomyces cerevisiae; *Saccharomyces cerevisiae Proteins; Schizosaccharomyces; Sequence Alignment; Sequence Homology, Amino Acid; Tumor Cells, Cultured; Zinc Fingers
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The zinc finger protein ZPR1 translocates from the cytoplasm to the nucleus after treatment of cells with mitogens. The function of nuclear ZPR1 has not been defined. Here we demonstrate that ZPR1 accumulates in the nucleolus of proliferating cells. The role of ZPR1 was examined using a gene disruption strategy. Cells lacking ZPR1 are not viable. Biochemical analysis demonstrated that the loss of ZPR1 caused disruption of nucleolar function, including preribosomal RNA expression. These data establish ZPR1 as an essential protein that is required for normal nucleolar function in proliferating cells.
Source
Mol Biol Cell. 1998 Oct;9(10):2963-71.
Journal/Book/Conference Title
Molecular biology of the cell
Related Resources
PubMed ID
9763455
Repository Citation
Galcheva-Gargova, Zoya; Gangwani, Laxman; Konstantinov, Konstantin N.; Mikrut, Monique; Theroux, Steven J.; Enoch, Tamar; and Davis, Roger J., "The cytoplasmic zinc finger protein ZPR1 accumulates in the nucleolus of proliferating cells" (1998). Open Access Articles. 1403.
https://escholarship.umassmed.edu/oapubs/1403