Department of Biochemistry and Molecular Pharmacology; Department of Physiology
Animals; Biological Transport; Cell Nucleolus; Cells, Cultured; Cytoplasm; Image Processing, Computer-Assisted; In Situ Hybridization; Microscopy, Video; Myoblasts; RNA, Ribosomal; Rats; Ribosomes
Life Sciences | Medicine and Health Sciences
Although the complex process of ribosome assembly in the nucleolus is beginning to be understood, little is known about how the ribosomal subunits move from the nucleolus to the nuclear membrane for transport to the cytoplasm. We show here that large ribosomal subunits move out from the nucleolus and into the nucleoplasm in all directions, with no evidence of concentrated movement along directed paths. Mobility was slowed compared with that expected in aqueous solution in a manner consistent with anomalous diffusion. Once nucleoplasmic, the subunits moved in the same random manner and also sometimes visited another nucleolus before leaving the nucleus.
DOI of Published Version
Mol Biol Cell. 2003 Dec;14(12):4805-12. Epub 2003 Sep 5. Link to article on publisher's site
Molecular biology of the cell
Politz JC, Tuft RA, Pederson T. (2003). Diffusion-based transport of nascent ribosomes in the nucleus. Open Access Publications by UMMS Authors. https://doi.org/10.1091/mbc.E03-06-0395. Retrieved from https://escholarship.umassmed.edu/oapubs/1390