Notch signaling plays a key role in cardiac cell differentiation
Department of Medicine and Cell Biology; Department of Physiology
Animals; Biological Markers; *Cell Differentiation; Chick Embryo; Gene Expression Regulation, Developmental; Heart; Myocardium; RNA, Messenger; Receptor, Notch1; *Signal Transduction
Life Sciences | Medicine and Health Sciences
Results from lineage tracing studies indicate that precursor cells in the ventricles give rise to both cardiac muscle and conduction cells. Cardiac conduction cells are specialized cells responsible for orchestrating the rhythmic contractions of the heart. Here, we show that Notch signaling plays an important role in the differentiation of cardiac muscle and conduction cell lineages in the ventricles. Notch1 expression coincides with a conduction marker, HNK-1, at early stages. Misexpression of constitutively active Notch1 (NIC) in early heart tubes in chick exhibited multiple effects on cardiac cell differentiation. Cells expressing NIC had a significant decrease in expression of cardiac muscle markers, but an increase in expression of conduction cell markers, HNK-1, and SNAP-25. However, the expression of the conduction marker connexin 40 was inhibited. Loss-of-function study, using a dominant-negative form of Suppressor-of-Hairless, further supports that Notch1 signaling is important for the differentiation of these cardiac cell types. Functional studies show that the expression of constitutively active Notch1 resulted in abnormalities in ventricular conduction pathway patterns.
DOI of Published Version
Mech Dev. 2006 Aug;123(8):626-40. Epub 2006 Jul 14. Link to article on publisher's site
Mechanisms of development
Chau, Mary D.; Tuft, Richard A.; Fogarty, Kevin E.; and Bao, Zheng-Zheng, "Notch signaling plays a key role in cardiac cell differentiation" (2006). Open Access Articles. 1375.