Title
Compartmentalized phosphorylation of IAP by protein kinase A regulates cytoprotection
UMMS Affiliation
Department of Cancer Biology
Publication Date
7-7-2007
Document Type
Article
Subjects
Animals; Apoptosis; *Cell Compartmentation; Cell Line, Tumor; Cyclic AMP-Dependent Protein Kinases; *Cytoprotection; Humans; Inhibitor of Apoptosis Proteins; Mice; Microtubule-Associated Proteins; Mitochondria; Neoplasms; Phosphorylation; Protein Structure, Secondary; Serine; Transplantation, Heterologous; X-Linked Inhibitor of Apoptosis Protein
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Cell death pathways are likely regulated in specialized subcellular microdomains, but how this occurs is not understood. Here, we show that cyclic AMP-dependent protein kinase A (PKA) phosphorylates the inhibitor of apoptosis (IAP) protein survivin on Ser20 in the cytosol, but not in mitochondria. This phosphorylation event disrupts the binding interface between survivin and its antiapoptotic cofactor, XIAP. Conversely, mitochondrial survivin or a non-PKA phosphorylatable survivin mutant binds XIAP avidly, enhances XIAP stability, synergistically inhibits apoptosis, and accelerates tumor growth, in vivo. Therefore, differential phosphorylation of survivin by PKA in subcellular microdomains regulates tumor cell apoptosis via its interaction with XIAP.
DOI of Published Version
10.1016/j.molcel.2007.06.004
Source
Mol Cell. 2007 Jul 6;27(1):17-28. Link to article on publisher's site
Journal/Book/Conference Title
Molecular cell
Related Resources
PubMed ID
17612487
Repository Citation
Dohi, Takehiko; Xia, Fang; and Altieri, Dario C., "Compartmentalized phosphorylation of IAP by protein kinase A regulates cytoprotection" (2007). Open Access Articles. 1327.
https://escholarship.umassmed.edu/oapubs/1327