UMMS Affiliation
Department of Physiology
Publication Date
2007-10-12
Document Type
Article
Subjects
Animals; Antibodies; Mice; Myosin Type II; NIH 3T3 Cells; Phosphorylation; Phosphoserine; Platelet-Derived Growth Factor; Protein Kinase C; Protein Kinase Inhibitors; Receptors, Platelet-Derived Growth Factor; Sensitivity and Specificity
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Phosphorylation of the regulatory light chain of myosin II (MLC(20)) at the activation sites promotes both the motor activity and the filament formation of myosin II, thus playing an important role in various cell motile processes. In contrast, the physiological function of phosphorylation of MLC(20) at the inhibitory sites is unknown. Here we report for the first time the function of the inhibitory site phosphorylation in the cells. We successfully produced the antibodies specifically recognizing the phosphorylation sites of MLC(20) at Ser1, and the platelet-derived growth factor (PDGF)-induced change in the phosphorylation at the Ser1 was monitored. The phosphorylation of MLC(20) at the Ser1 significantly increased during the PDGF-induced actin cytoskeletal reorganization. PDGF disassembled the stress fibers, and this was attenuated with the expression of unphosphorylatable MLC(20) at the Ser1/Ser2 phosphorylation sites. The present results suggest that the down-regulation of myosin II activity achieved by the phosphorylation at the Ser1/Ser2 sites plays an important role in the normal reorganization of actomyosin filaments triggered by PDGF receptor stimulation.
DOI of Published Version
10.1091/mbc.E06-12-1076
Source
Mol Biol Cell. 2007 Dec;18(12):5081-90. Epub 2007 Oct 10. Link to article on publisher's site
Journal/Book/Conference Title
Molecular biology of the cell
Related Resources
PubMed ID
17928407
Repository Citation
Komatsu S, Ikebe M. (2007). The phosphorylation of myosin II at the Ser1 and Ser2 is critical for normal platelet-derived growth factor induced reorganization of myosin filaments. Open Access Articles. https://doi.org/10.1091/mbc.E06-12-1076. Retrieved from https://escholarship.umassmed.edu/oapubs/1319