Department of Medicine, Division of Infectious Diseases and Immunology
Blood Bactericidal Activity; Colony Count, Microbial; Complement C4b-Binding Protein; Galactosyltransferases; Heptoses; Humans; Lipopolysaccharides; Mutation; Neisseria gonorrhoeae; Porins; Protein Binding; Serum
Life Sciences | Medicine and Health Sciences
Lipooligosaccharide (LOS) heptose (Hep) glycan substitutions influence gonococcal serum resistance. Several gonococcal strains bind the classical complement pathway inhibitor, C4b-binding protein (C4BP), via their porin (Por) molecule to escape complement-dependent killing by normal human serum (NHS). We show that the proximal glucose (Glc) on HepI is required for C4BP binding to Por1B-bearing gonococcal strains MS11 and 1291 but not to FA19 (Por1A). The presence of only the proximal Glc on HepI (lgtE mutant) permitted maximal C4BP binding to MS11 but not to 1291. Replacing 1291 lgtE Por with MS11 Por increased C4BP binding to levels that paralleled MS11 lgtE, suggesting that replacement of the Por1B molecule dictated the effects of HepI glycans on C4BP binding. The remainder of the strain background did not affect C4BP binding; replacing the Por of strain F62 with MS11 Por (F62 PorMS11) and truncating HepI mirrored the findings in the MS11 background. C4BP binding correlated with resistance to killing by NHS in most instances. F62 PorMS11 and its lgtE mutant were sensitive to NHS despite binding C4BP, secondary to kinetically overwhelming classical pathway activation and possibly increased alternative pathway activation (measured by factor Bb binding) by the F62 background. FA19 lgtF (HepI unsubstituted) resisted killing by only 10% NHS, not 50% NHS, despite binding levels of C4BP similar to those of FA19 and FA19 lgtE (both resistant to 50% serum), suggesting a role for the proximal Glc in serum resistance independently of C4BP binding. This study provides mechanistic insights into how HepI LOS substitutions affect the serum resistance of N. gonorrhoeae.
DOI of Published Version
Infect Immun. 2007 Aug;75(8):4071-81. Epub 2007 May 25. Link to article on publisher's site
Infection and immunity
Ram, Sanjay; Ngampasutadol, Jutamas; Cox, Andrew D.; Blom, Anna M.; Lewis, Lisa A.; St. Michael, Frank; Stupak, Jacek; Gulati, Sunita; and Rice, Peter A., "Heptose I glycan substitutions on Neisseria gonorrhoeae lipooligosaccharide influence C4b-binding protein binding and serum resistance" (2007). Open Access Articles. 1263.