Phosphoinositide-dependent pathways in mouse sperm are regulated by egg ZP3 and drive the acrosome reaction

UMMS Affiliation

Department of Cell Biology

Publication Date


Document Type



Acrosome Reaction; Animals; Egg Proteins; Female; Fluorescent Antibody Technique; Immunoblotting; Lipids; Male; Membrane Glycoproteins; Mice; Phosphatidylinositol Phosphates; Receptors, Cell Surface; Signal Transduction; Spermatozoa


Cell Biology | Life Sciences | Medicine and Health Sciences


Sperm of many animals must complete an exocytotic event, the acrosome reaction, in order to fuse with eggs. In mammals, acrosome reactions are triggered during sperm contact with the egg extracellular matrix, or zona pellucida, by the matrix glycoprotein ZP3. Here, we show that ZP3 stimulates production of phosphatidylinositol-(3,4,5)-triphosphate in sperm membranes. Phosphatidylinositol-3-kinase antagonists that prevent acrosome reactions and fertilization in vitro, while generation of this phosphoinositide in the absence of ZP3 triggered acrosome reactions. Downstream effectors of phosphatidylinositol-(3,4,5)-triphosphate in sperm include the protein kinases, Akt and PKCzeta. These studies outline a signal transduction pathway that plays an essential role in the early events of mammalian fertilization.

DOI of Published Version



Dev Biol. 2007 Apr 1;304(1):116-26. Epub 2006 Dec 15. Link to article on publisher's site

Journal/Book/Conference Title

Developmental biology

Related Resources

Link to Article in PubMed

PubMed ID