Title
Involvement of mast cells in basal and neurotensin-induced intestinal absorption of taurocholate in rats
UMMS Affiliation
Department of Physiology
Publication Date
2003-12-25
Document Type
Article
Subjects
Animals; Duodenum; Intestinal Absorption; Intestines; Jejunum; Male; Mast Cells; Neurotensin; Rats; Rats, Sprague-Dawley; Taurocholic Acid
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Neurotensin (NT), a hormone released from intestine by ingested fat, facilitates lipid digestion by stimulating pancreatic secretion and slowing the movement of chyme. In addition, NT can contract the gall bladder and enhance the enterohepatic circulation (EHC) of bile acids to promote micelle formation. Our recent finding that NT enhanced and an NT antagonist inhibited [(3)H]taurocholate ([(3)H]TC) absorption from proximal rat small intestine indicated a role for endogenous NT in the regulation of EHC. Here, we postulate the involvement of intestinal mast cells in the TC uptake process and in the stimulatory effect of NT. In anesthetized rats with the bile duct cannulated for bile collection, infusion of NT (10 pmol.kg(-1).min(-1)) enhanced the [(3)H]TC recovery rate from duodenojejunum by 2.2-fold. This response was abolished by pretreatment with mast cell stabilizers (cromoglycate, doxantrazole) and inhibitors of mast cell mediators (diphenhydramine, metergoline, zileuton). In contrast, mast cell degranulators (compound 48/80, substance P) and mast cell mediators (histamine, leukotriene C(4)) reproduced the effect of NT. N(G)-nitro-l-arginine methyl ester enhanced and l-arginine inhibited basal and NT-induced TC uptake, consistent with the known inhibitory effect of nitric oxide (NO) on mast cell reactivity. These results argue that basal and NT-stimulated TC uptake in rat jejunum are similarly dependent on mast cells, are largely mediated by release of mast cell mediators, and are subject to regulation by NO.
DOI of Published Version
10.1152/ajpgi.00178.2003
Source
Am J Physiol Gastrointest Liver Physiol. 2004 Aug;287(2):G408-16. Link to article on publisher's site
Journal/Book/Conference Title
American journal of physiology. Gastrointestinal and liver physiology
Related Resources
PubMed ID
14693504
Repository Citation
Gui, Xianyong and Carraway, Robert E., "Involvement of mast cells in basal and neurotensin-induced intestinal absorption of taurocholate in rats" (2003). Open Access Articles. 118.
https://escholarship.umassmed.edu/oapubs/118