MD-2 expression is not required for cell surface targeting of Toll-like receptor 4 (TLR4)

UMMS Affiliation

Department of Medicine, Division of Infectious Diseases and Immunology

Publication Date


Document Type



Animals; Cell Line; *Gene Expression Regulation; Humans; Lipopolysaccharides; Lymphocyte Antigen 96; Mice; Mice, Knockout; Toll-Like Receptor 4


Life Sciences | Medicine and Health Sciences


The cell surface receptor complex formed by TLR4 and myeloid differentiation 2 (MD-2) is engaged when cells are exposed to LPS. Recent studies suggested that surface localization of functional mouse TLR4 (mTLR4) depends on the simultaneous expression of MD-2. As we did not observe a similar requirement, we conducted a comparative study of human TLR4 and mTLR4 surface expression in immune cells derived from the MD-2 knockout mouse and LPS-responsive cell lines and in cells that ectopically express TLR4. Our results indicate that in the human and mouse models, neither TLR4 function nor TLR4 surface targeting requires MD-2 coexpression. Accordingly, we report on one human cell line, which constitutively expresses functional TLR4 on the cell surface in the absence of MD-2 expression.

DOI of Published Version



J Leukoc Biol. 2006 Dec;80(6):1584-92. Epub 2006 Aug 31. Link to article on publisher's site

Journal/Book/Conference Title

Journal of leukocyte biology

Related Resources

Link to Article in PubMed

PubMed ID