The role of CD40-CD154 interaction in antiviral T cell-independent IgG responses
Department of Pathology
Animals; Antibodies, Viral; Antigens, CD40; Antigens, T-Independent; B-Lymphocytes; CD40 Ligand; Capsid; *Capsid Proteins; Genes, T-Cell Receptor beta; Genes, T-Cell Receptor delta; Immunoglobulin G; Ligands; Lymphopenia; Membrane Glycoproteins; Mice; Mice, Inbred C57BL; Mice, Knockout; Mice, SCID; Polyomavirus; Polyomavirus Infections; T-Lymphocytes; Tumor Virus Infections
Life Sciences | Medicine and Health Sciences
Polyomavirus (PyV) infection elicits protective T cell-independent (TI) IgG responses in T cell-deficient mice. The question addressed in this report is whether CD40 signaling plays a role in this TI antiviral IgG response. Because CD40 ligand (CD40L) can be expressed on numerous cell types in addition to activated T cells, it is possible that cells other than T cells provide CD40L to signal through CD40 on B cells and hence positively influence the antiviral TI IgG responses. In this study we show, by blocking CD40-CD40L interactions in vivo with anti-CD40L Ab treatment in TCR betaxdelta-/- mice and by using SCID mice reconstituted with CD40-/- B cells, that the lack of CD40 signaling in B cells results in a 50% decrease in TI IgG secreted in response to PyV. SCID mice reconstituted with CD40L-/- B cells also responded to PyV infection with diminished IgG secretion compared with that of SCID mice reconstituted with wild-type B cells. This finding suggests that B cells may provide the CD40L for CD40 signaling in the absence of T cell help during acute virus infection. Our studies demonstrate that, although about half of the TI IgG responses to PyV are independent of CD40-CD40L interactions, these interactions occur in T cell-deficient mice and enhance antiviral TI Ab responses.
DOI of Published Version
J Immunol. 2000 Jun 1;164(11):5877-82.
Journal of immunology (Baltimore, Md. : 1950)
Szomolanyi-Tsuda, Eva; Brien, James D.; Dorgan, Jill E.; Welsh, Raymond M.; and Garcea, Robert L., "The role of CD40-CD154 interaction in antiviral T cell-independent IgG responses" (2000). Open Access Articles. 1132.