Differential kinetics and specificity of EBV-specific CD4+ and CD8+ T cells during primary infection

UMMS Affiliation

Graduate Program in Immunology/Virology; Department of Pediatrics

Publication Date


Document Type



Acute Disease; Adult; Amino Acid Sequence; Antigen Presentation; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Cell Division; Epitopes, T-Lymphocyte; Epstein-Barr Virus Nuclear Antigens; Herpesvirus 4, Human; Humans; Infectious Mononucleosis; Lymphocyte Count; Molecular Sequence Data; Nuclear Proteins; Phosphoproteins; Trans-Activators; Transcription Factors; Viral Load; *Viral Proteins


Life Sciences | Medicine and Health Sciences


The generation and maintenance of virus-specific CD4(+) T cells in humans are not well understood. We used short in vitro stimulation assays followed by intracellular cytokine staining to characterize the timing, magnitude, and Ag specificity of CD4(+) T cells over the course of primary EBV infection. Lytic and latent protein-specific CD4(+) T cells were readily detected at presentation with acute infectious mononucleosis and declined rapidly thereafter. Responses to BZLF-1, BMLF-1, and Epstein-Barr nuclear Ag-3A were more commonly detected than responses to Epstein-Barr nuclear Ag-1. Concurrent analyses of BZLF-1-specific CD4(+) and CD8(+) T cells revealed differences in the expansion, specificity, and stability of CD4(+) and CD8(+) T cell-mediated responses over time. Peripheral blood EBV load directly correlated with the frequency of EBV-specific CD4(+) T cell responses at presentation and over time, suggesting that EBV-specific CD4(+) T cell responses are Ag-driven.

DOI of Published Version



J Immunol. 2003 Mar 1;170(5):2590-8.

Journal/Book/Conference Title

Journal of immunology (Baltimore, Md. : 1950)

Related Resources

Link to Article in PubMed

PubMed ID