Cutting edge: an alternative pathway of CD4+ T cell differentiation is induced following activation in the absence of gamma-chain-dependent cytokine signals
Department of Pathology
Animals; CD4-Positive T-Lymphocytes; *Cell Differentiation; Cell Proliferation; Cells, Cultured; Cytokines; DNA-Binding Proteins; Homeostasis; Janus Kinase 3; Lymphocyte Activation; Mice; Mice, Inbred C57BL; Mice, Knockout; Phenotype; Protein-Tyrosine Kinases; *Signal Transduction; Thymus Gland
Life Sciences | Medicine and Health Sciences
This report addresses the role of gamma-chain cytokine signals in regulating CD4(+) T cell differentiation following activation. Using murine CD4(+) T cells lacking the Jak3 tyrosine kinase, we show that activation of these cells in the absence of gamma-chain-dependent cytokine signals induces an alternative pathway of T cell differentiation. Specifically, activated Jak3(-/-) CD4(+) T cells produce IL-10, TGF-beta, and IFN-gamma, but not IL-2 or IL-4, and are unable to proliferate in vitro. In addition, Jak3(-/-) CD4(+) T cells express high levels of programmed death-1 and lymphocyte activation gene-3 and modestly suppress the proliferation of wild-type CD4(+) T cells in coculture assays. Together, these features demonstrate a striking similarity between Jak3(-/-) CD4(+) T cells and the regulatory T cells that have been shown to suppress immune responses in vitro and in vivo. We conclude that Jak3 is a critical component of signaling pathways that regulate T cell differentiation into effector vs regulatory lineages.
DOI of Published Version
J Immunol. 2006 Feb 15;176(4):2059-63.
Journal of immunology (Baltimore, Md. : 1950)
Mayack, Shane Renee and Berg, Leslie J., "Cutting edge: an alternative pathway of CD4+ T cell differentiation is induced following activation in the absence of gamma-chain-dependent cytokine signals" (2006). Open Access Articles. 1088.