Title

Identification and analysis for cross-reactivity among hantaviruses of H-2b-restricted cytotoxic T-lymphocyte epitopes in Sin Nombre virus nucleocapsid protein

UMMS Affiliation

Center for Infectious Disease and Vaccine Research

Publication Date

6-26-2004

Document Type

Article

Subjects

Amino Acid Sequence; Animals; Antibody Specificity; Cell Line, Tumor; Cross Reactions; Epitopes; H-2 Antigens; Hantavirus; Hantavirus Pulmonary Syndrome; Humans; Isoantigens; Major Histocompatibility Complex; Mice; Molecular Sequence Data; Nucleocapsid; Nucleocapsid Proteins; Peptide Fragments; Sin Nombre virus; T-Lymphocytes, Cytotoxic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Sin Nombre virus (SNV) causes hantavirus pulmonary syndrome (HPS), with a high rate of mortality in humans who are infected by the transmission of virus from the natural rodent host. In humans, cytotoxic T lymphocytes (CTL) specific for SNV appear to play an important role in the pathogenicity of HPS. There is a correlation between the frequencies of SNV-specific CTLs and the severity of HPS disease. In order to create a mouse model to study the role of SNV-specific T cells in vivo, T cell responses to SNV nucleocapsid (N) protein in B6.PL Thy1(a)/Cy mice (H-2(b)) immunized with plasmid DNA or recombinant vaccinia virus expressing SNV N protein were examined. Four peptides, NC94-101, NC175-189, NC217-231 and NC331-345, were recognized by CD8(+) T cells in CTL and ELISPOT assays in SNV N-immunized mice. Interestingly, two of these epitopes are located in the central region of the SNV N protein, where several human CD8(+) T-cell epitopes have been defined in Puumala virus and SNV. CTL lines specific for these four epitopes were cross-reactive to corresponding Puumala virus peptides, but only one of them was cross-reactive to Hantaan virus peptides. These results will enable the analysis of the roles of CTL in immunopathology of HPS in experimental mouse models of HPS.

DOI of Published Version

10.1099/vir.0.79945-0

Source

J Gen Virol. 2004 Jul;85(Pt 7):1909-19. Link to article on publisher's site

Journal/Book/Conference Title

The Journal of general virology

Related Resources

Link to Article in PubMed

PubMed ID

15218176

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