UMMS Affiliation

Department of Hospital Laboratories

Publication Date


Document Type



Bacteremia; HIV Seropositivity; Humans; Mycobacterium avium Complex


Medical Microbiology


Recovery of Mycobacterium avium complex organisms from 412 lysis-centrifugation (Isolator) concentrates of blood specimens obtained from human immunodeficiency virus-positive individuals was attempted with the following media (and Isolator concentrate inoculum volumes): BACTEC 12B broth (0.2 and 1.0 ml), Lowenstein-Jensen slants (0.1 ml), and Middlebrook 7H10/11 agar (0.1 ml). A total of 42 M. avium complex isolates were recovered. The highest rates of recovery and shortest detection times were noted with BACTEC 12B bottles inoculated with 0.2 ml of Isolator concentrate. Middlebrook agar was superior to Lowenstein-Jensen slants. Fluorochrome acid-fast smears performed directly upon Isolator concentrates were of no utility.


J Clin Microbiol. 1994 Oct;32(10):2576-7.

Journal/Book/Conference Title

Journal of clinical microbiology

Related Resources

Link to Article in PubMed

PubMed ID




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