Department of Neurobiology; Budnik Lab
Animals; Cell Nucleus; Drosophila melanogaster; Ecdysone; *Gene Expression Regulation, Developmental; Lamin Type A; Muscles; Signal Transduction
Neuroscience and Neurobiology
The inner side of the nuclear envelope (NE) is lined with lamins, a meshwork of intermediate filaments that provides structural support for the nucleus and plays roles in many nuclear processes. Lamins, classified as A- or B-types on the basis of biochemical properties, have a conserved globular head, central rod and C-terminal domain that includes an Ig-fold structural motif. In humans, mutations in A-type lamins give rise to diseases that exhibit tissue-specific defects, such as Emery-Dreifuss muscular dystrophy. Drosophila is being used as a model to determine tissue-specific functions of A-type lamins in development, with implications for understanding human disease mechanisms. The GAL4-UAS system was used to express wild-type and mutant forms of Lamin C (the presumed Drosophila A-type lamin), in an otherwise wild-type background. Larval muscle-specific expression of wild type Drosophila Lamin C caused no overt phenotype. By contrast, larval muscle-specific expression of a truncated form of Lamin C lacking the N-terminal head (Lamin C DeltaN) caused muscle defects and semi-lethality, with adult 'escapers' possessing malformed legs. The leg defects were due to a lack of larval muscle function and alterations in hormone-regulated gene expression. The consequences of Lamin C association at a gene were tested directly by targeting a Lamin C DNA-binding domain fusion protein upstream of a reporter gene. Association of Lamin C correlated with localization of the reporter gene at the nuclear periphery and gene repression. These data demonstrate connections among the Drosophila A-type lamin, hormone-induced gene expression and muscle function.
Rights and Permissions
© 2010. Published by The Company of Biologists Ltd. Publisher PDF posted as allowed by the publisher's author rights policy at http://dev.biologists.org/site/misc/rights_permissions.xhtml#author.
DOI of Published Version
Development. 2010 Sep;137(18):3067-77. Epub 2010 Aug 11. Link to article on publisher's site
Development (Cambridge, England)
Dialynas G, Speese SD, Budnik V, Geyer PK, Wallrath LL. (2010). The role of Drosophila Lamin C in muscle function and gene expression. Neurobiology Publications. https://doi.org/10.1242/dev.048231. Retrieved from https://escholarship.umassmed.edu/neurobiology_pp/8