Sleep rhythmicity and homeostasis in mice with targeted disruption of mPeriod genes

UMMS Affiliation

Department of Neurobiology; Weaver Lab

Publication Date


Document Type



Alleles; Animals; Body Temperature; Cell Cycle Proteins; Circadian Rhythm; Electrodes, Implanted; Electroencephalography; Electromyography; Genotype; Homeostasis; Male; Mice; Motor Activity; Mutation; Nuclear Proteins; Period Circadian Proteins; *Periodicity; Reverse Transcriptase Polymerase Chain Reaction; Sleep; Sleep, REM; Transcription Factors; Wakefulness


Neuroscience and Neurobiology


In mammals, sleep is regulated by circadian and homeostatic mechanisms. The circadian component, residing in the suprachiasmatic nucleus (SCN), regulates the timing of sleep, whereas homeostatic factors determine the amount of sleep. It is believed that these two processes regulating sleep are independent because sleep amount is unchanged after SCN lesions. However, because such lesions necessarily damage neuronal connectivity, it is preferable to investigate this question in a genetic model that overcomes the confounding influence of circadian rhythmicity. Mice with disruption of both mouse Period genes (mPer)1 and mPer2 have a robust diurnal sleep-wake rhythm in an entrained light-dark cycle but lose rhythmicity in a free-run condition. Here, we examine the role of the mPer genes on the rhythmic and homeostatic regulation of sleep. In entrained conditions, when averaged over the 24-h period, there were no significant differences in waking, slow-wave sleep (SWS), or rapid eye movement (REM) sleep between mPer1, mPer2, mPer3, mPer1-mPer2 double-mutant, and wild-type mice. The mice were then kept awake for 6 h (light period 6-12), and the mPer mutants exhibited increased sleep drive, indicating an intact sleep homeostatic response in the absence of the mPer genes. In free-run conditions (constant darkness), the mPer1-mPer2 double mutants became arrhythmic, but they continued to maintain their sleep levels even after 36 days in free-running conditions. Although mPer1 and mPer2 represent key elements of the molecular clock in the SCN, they are not required for homeostatic regulation of the daily amounts of waking, SWS, or REM sleep.

DOI of Published Version



Am J Physiol Regul Integr Comp Physiol. 2004 Jul;287(1):R47-57. Epub 2004 Mar 18. Link to article on publisher's website

Journal/Book/Conference Title

American journal of physiology. Regulatory, integrative and comparative physiology 15191924

Related Resources

Link to Article in PubMed

PubMed ID