In Situ Regulated Dopamine Transporter Trafficking: There's No Place Like Home
Brudnick Neuropsychiatric Research Institute, Department of Neurobiology; Graduate School of Biomedical Sciences, Neuroscience Program; Melikian Lab
Neuroscience and Neurobiology
Dopamine (DA) is critical for motivation, reward, movement initiation, and learning. Mechanisms that control DA signaling have a profound impact on these important behaviors, and additionally play a role in DA-related neuropathologies. The presynaptic SLC6 DA transporter (DAT) limits extracellular DA levels by clearing released DA, and is potently inhibited by addictive and therapeutic psychostimulants. Decades of evidence support that the DAT is subject to acute regulation by a number of signaling pathways, and that endocytic trafficking strongly regulates DAT availability and function. DAT trafficking studies have been performed in a variety of model systems, including both in vitro and ex vivo preparations. In this review, we focus on the breadth of DAT trafficking studies, with specific attention to, and comparison of, how context may influence DAT's response to different stimuli. In particular, this overview highlights that stimulated DAT trafficking not only differs between in vitro and ex vivo environments, but also is influenced by both sex and anatomical subregions.
Dopamine transporter, Membrane trafficking, Striatum
DOI of Published Version
Fagan RR, Kearney PJ, Melikian HE. In Situ Regulated Dopamine Transporter Trafficking: There's No Place Like Home. Neurochem Res. 2020 Mar 7. doi: 10.1007/s11064-020-03001-6. Epub ahead of print. PMID: 32146647. Link to article on publisher's site
Fagan RR, Kearney PJ, Melikian HE. (2020). In Situ Regulated Dopamine Transporter Trafficking: There's No Place Like Home. Neurobiology Publications. https://doi.org/10.1007/s11064-020-03001-6. Retrieved from https://escholarship.umassmed.edu/neurobiology_pp/259