Department of Neurobiology; Alkema Lab
Genetics and Genomics | Neuroscience and Neurobiology
Selenium is a trace element for most organisms; its deficiency and excess are detrimental. Selenium beneficial effects are mainly due to the role of the 21(st) genetically encoded amino acid selenocysteine (Sec). Selenium also exerts Sec-independent beneficial effects. Its harmful effects are thought to be mainly due to non-specific incorporation in protein synthesis. Yet the selenium response in animals is poorly understood. In Caenorhabditis elegans, Sec is genetically incorporated into a single selenoprotein. Similar to mammals, a 20-fold excess of the optimal selenium requirement is harmful. Sodium selenite (Na2SeO3) excess causes development retardation, impaired growth, and neurodegeneration of motor neurons. To study the organismal response to selenium we performed a genetic screen for C. elegans mutants that are resistant to selenite. We isolated non-sense and missense egl-9/EGLN mutants that confer robust resistance to selenium. In contrast, hif-1/HIF null mutant was highly sensitive to selenium, establishing a role for this transcription factor in the selenium response. We showed that EGL-9 regulates HIF-1 activity through VHL-1, and identified CYSL-1 as a key sensor that transduces the selenium signal. Finally, we showed that the key enzymes involved in sulfide and sulfite stress (sulfide quinone oxidoreductase and sulfite oxidase) are not required for selenium resistance. In contrast, knockout strains in the persulfide dioxygenase ETHE-1 and the sulfurtransferase MPST-7 affect the organismal response to selenium. In sum, our results identified a transcriptional pathway as well as enzymes possibly involved in the organismal selenium response.
CYSL-1, Caenorhabditis elegans, EGL-9, HIF-1, selenite, selenium, stress, sulfide
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Copyright © 2020 Romanelli-Credrez, Doitsidou, Alkema and Salinas. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
DOI of Published Version
Romanelli-Credrez L, Doitsidou M, Alkema MJ, Salinas G. HIF-1 Has a Central Role in Caenorhabditis elegans Organismal Response to Selenium. Front Genet. 2020 Feb 25;11:63. doi: 10.3389/fgene.2020.00063. PMID: 32161616; PMCID: PMC7052493. Link to article on publisher's site
Frontiers in genetics
Romanelli-Credrez L, Doitsidou M, Alkema MJ, Salinas G. (2020). HIF-1 Has a Central Role in Caenorhabditis elegans Organismal Response to Selenium. Neurobiology Publications. https://doi.org/10.3389/fgene.2020.00063. Retrieved from https://escholarship.umassmed.edu/neurobiology_pp/256
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This work is licensed under a Creative Commons Attribution 4.0 License.