Title

Targeted Complement Inhibition at Synapses Prevents Microglial Synaptic Engulfment and Synapse Loss in Demyelinating Disease

UMMS Affiliation

Department of Neurobiology, Brudnik Neuropsychiatric Research Institute; Horae Gene Therapy Center; Li Weibo Institute for Rare Diseases Research; Department of Microbiologic and Physiological Systems; Schafer Lab

Publication Date

2020-01-14

Document Type

Article

Disciplines

Immunity | Nervous System | Nervous System Diseases | Neuroscience and Neurobiology

Abstract

Multiple sclerosis (MS) is a demyelinating, autoimmune disease of the central nervous system. While work has focused on myelin and axon loss in MS, less is known about mechanisms underlying synaptic changes. Using postmortem human MS tissue, a preclinical nonhuman primate model of MS, and two rodent models of demyelinating disease, we investigated synapse changes in the visual system. Similar to other neurodegenerative diseases, microglial synaptic engulfment and profound synapse loss were observed. In mice, synapse loss occurred independently of local demyelination and neuronal degeneration but coincided with gliosis and increased complement component C3, but not C1q, at synapses. Viral overexpression of the complement inhibitor Crry at C3-bound synapses decreased microglial engulfment of synapses and protected visual function. These results indicate that microglia eliminate synapses through the alternative complement cascade in demyelinating disease and identify a strategy to prevent synapse loss that may be broadly applicable to other neurodegenerative diseases. VIDEO ABSTRACT.

Keywords

complement, demyelination, engulfment, gene therapy, microglia, multiple sclerosis, neural-immune, neurodegeneration, neuroinflammation, synapse

DOI of Published Version

10.1016/j.immuni.2019.12.004

Source

Immunity. 2020 Jan 14;52(1):167-182.e7. doi: 10.1016/j.immuni.2019.12.004. Epub 2019 Dec 26. Link to article on publisher's site

Journal/Book/Conference Title

Immunity

Related Resources

Link to Article in PubMed

PubMed ID

31883839

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