Department of Neurobiology; Weaver Lab
Neuroscience and Neurobiology
Daily torpor is used by small mammals to reduce daily energy expenditure in response to energetic challenges. Optimizing the timing of daily torpor allows mammals to maximize its energetic benefits and, accordingly, torpor typically occurs in the late night and early morning in most species. The regulatory mechanisms underlying such temporal regulation have however not been elucidated. Direct control by the circadian clock and indirect control through the timing of food intake have both been suggested as possible mechanisms. Here, feeding cycles outside of the circadian range and brain-specific mutations of circadian clock genes (Vgat-Cre(+)CK1delta(fl/fl)(fl/+); Vgat-Cre(+)Bmal1(fl/fl) ) were used to separate the roles of the circadian clock and food timing in controlling the timing of daily torpor in mice. These experiments revealed that the timing of daily torpor is transiently inhibited by feeding, while the circadian clock is the major determinant of the timing of torpor. Torpor never occurred during the early part of the circadian active phase, but is preferentially initiated late in the subjective night. Food intake disrupted torpor in the first 4-6 h after feeding by preventing or interrupting torpor bouts. Following interruption, re-initiation of torpor was unlikely until after the next circadian active phase. Overall, these results demonstrate that feeding transiently inhibits torpor while the central circadian clock gates the timing of daily torpor in response to energetic challenges by restricting the initiation of torpor to a specific circadian phase.
Body temperature, Circadian rhythm, Clock mutant, Energetic savings, Metabolism, Suprachiasmatic nucleus
Rights and Permissions
© 2018. Published by The Company of Biologists Ltd. Accepted manuscript posted after 12 months as allowed by the publisher's license at http://www.biologists.com/user-licence-1-1/.
DOI of Published Version
J Exp Biol. 2018 Jun 14. pii: jeb.179812. doi: 10.1242/jeb.179812. [Epub ahead of print] Link to article on publisher's site
The Journal of experimental biology
van der Vinne, Vincent; Bingaman, Mark J.; Weaver, David R.; and Swoap, Steven J., "Clocks and meals keep mice from being cool" (2018). Neurobiology Publications and Presentations. 224.