Dissection of Drosophila larval body-wall muscles
Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program
Animals; Dissection; Drosophila melanogaster; Larva; Magnetics; Muscles
Developmental Neuroscience | Molecular and Cellular Neuroscience
Over the last two decades, the Drosophila larval neuromuscular junction has gained immense popularity as a model system for the study of synaptic development, function, and plasticity. With this model, it is easy to visualize synapses and manipulate the system genetically with a high degree of temporal and spatial control, which makes it ideal for resolving problems in synaptic physiology and development. A number of different techniques have been used to dissect third-instar larval preparations to expose the body-wall muscles. Here, we describe a procedure that uses magnetic chambers and pins to allow for fine control in spreading the larval body wall.
DOI of Published Version
Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5469. doi: 10.1101/pdb.prot5469.
Cold Spring Harbor protocols
Ramachandran P, Budnik V. (2010). Dissection of Drosophila larval body-wall muscles. Neurobiology Publications and Presentations. https://doi.org/10.1101/pdb.prot5469. Retrieved from https://escholarship.umassmed.edu/neurobiology_pp/14