Title
Immunocytochemical staining of Drosophila larval body-wall muscles
UMMS Affiliation
Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program
Publication Date
2010-08-01
Document Type
Article
Subjects
Animals; Dissection; Drosophila melanogaster; Immunohistochemistry; Larva; Muscles; Staining and Labeling
Disciplines
Neuroscience and Neurobiology
Abstract
Over the last two decades, the Drosophila larval neuromuscular junction (NMJ) has gained immense popularity as a model system for the study of synaptic development, function, and plasticity. With this model, it is easy to visualize synapses and manipulate the system genetically with a high degree of temporal and spatial control, which makes it ideal for resolving problems in synaptic physiology and development. This article describes a procedure for labeling various proteins with antibodies in dissected larval body-wall muscles and visualizing their localization and distribution in the brain, NMJ, and muscle.
DOI of Published Version
10.1101/pdb.prot5470
Source
Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5470. doi: 10.1101/pdb.prot5470.
Journal/Book/Conference Title
Cold Spring Harbor protocols
Related Resources
PubMed ID
20679379
Repository Citation
Ramachandran P, Budnik V. (2010). Immunocytochemical staining of Drosophila larval body-wall muscles. Neurobiology Publications and Presentations. https://doi.org/10.1101/pdb.prot5470. Retrieved from https://escholarship.umassmed.edu/neurobiology_pp/13
Comments
First author Preethi Ramachandran is a doctoral student in the Neuroscience Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.