UMMS Affiliation

Department of Neurobiology; Weaver Lab

Publication Date


Document Type



Antibodies; CLOCK Proteins; Circadian Clocks; Suprachiasmatic Nucleus


Neuroscience and Neurobiology


Research on the mechanisms underlying circadian rhythmicity and the response of brain and body clocks to environmental and physiological challenges requires assessing levels of circadian clock proteins. Too often, however, it is difficult to acquire antibodies that specifically and reliably label these proteins. Many of these antibodies also lack appropriate validation. The goal of this project was to generate and characterize antibodies against several circadian clock proteins. We examined mice and hamsters at peak and trough times of clock protein expression in the suprachiasmatic nucleus (SCN). In addition, we confirmed specificity by testing the antibodies on mice with targeted disruption of the relevant genes. Our results identify antibodies against PER1, PER2, BMAL1 and CLOCK that are useful for assessing circadian clock proteins in the SCN by immunocytochemistry.

Rights and Permissions

Copyright: © 2012 LeSauter et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

DOI of Published Version



LeSauter J, Lambert CM, Robotham MR, Model Z, Silver R, et al. (2012) Antibodies for Assessing Circadian Clock Proteins in the Rodent Suprachiasmatic Nucleus. PLoS ONE 7(4): e35938. doi:10.1371/journal.pone.0035938. Link to article on publisher's site

Journal/Book/Conference Title

PloS one

Related Resources

Link to Article in PubMed

PubMed ID




To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.