UMMS Affiliation
Department of Neurobiology; Weaver Lab
Publication Date
2012-04-27
Document Type
Article
Subjects
Antibodies; CLOCK Proteins; Circadian Clocks; Suprachiasmatic Nucleus
Disciplines
Neuroscience and Neurobiology
Abstract
Research on the mechanisms underlying circadian rhythmicity and the response of brain and body clocks to environmental and physiological challenges requires assessing levels of circadian clock proteins. Too often, however, it is difficult to acquire antibodies that specifically and reliably label these proteins. Many of these antibodies also lack appropriate validation. The goal of this project was to generate and characterize antibodies against several circadian clock proteins. We examined mice and hamsters at peak and trough times of clock protein expression in the suprachiasmatic nucleus (SCN). In addition, we confirmed specificity by testing the antibodies on mice with targeted disruption of the relevant genes. Our results identify antibodies against PER1, PER2, BMAL1 and CLOCK that are useful for assessing circadian clock proteins in the SCN by immunocytochemistry.
Rights and Permissions
Copyright: © 2012 LeSauter et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI of Published Version
10.1371/journal.pone.0035938
Source
LeSauter J, Lambert CM, Robotham MR, Model Z, Silver R, et al. (2012) Antibodies for Assessing Circadian Clock Proteins in the Rodent Suprachiasmatic Nucleus. PLoS ONE 7(4): e35938. doi:10.1371/journal.pone.0035938. Link to article on publisher's site
Journal/Book/Conference Title
PloS one
Related Resources
PubMed ID
22558277
Repository Citation
Lesauter J, Lambert CM, Robotham MR, Model Z, Silver R, Weaver DR. (2012). Antibodies for assessing circadian clock proteins in the rodent suprachiasmatic nucleus. Neurobiology Publications and Presentations. https://doi.org/10.1371/journal.pone.0035938. Retrieved from https://escholarship.umassmed.edu/neurobiology_pp/113