Title
Separating changes in the intra- and extracellular water apparent diffusion coefficient following focal cerebral ischemia in the rat brain
UMMS Affiliation
Department of Neurology; Department of Radiology; Graduate School of Biomedical Sciences
Publication Date
2002-11
Document Type
Article
Subjects
Animals; Brain Ischemia; Diffusion Magnetic Resonance Imaging; Extracellular Space; Intracellular Fluid; Male; Rats; Rats, Sprague-Dawley; Spectrum Analysis; Water
Disciplines
Neurology | Neuroscience and Neurobiology
Abstract
Selective intracellular (IC) and extracellular (EC) brain water apparent diffusion coefficient (ADC) values were measured in normal and ischemic rat brain. Selective T(1)-relaxation enhancement of the EC water, using intracerebroventricular (ICV) infusion of an NMR contrast reagent (CR), was used to separate the IC and EC signal contributions. In the CR-infused, normal brain (n = 4), T(1) = 235 +/- 10 ms and T(2) = 46 +/- 2 ms for IC water (85%) and T(1) = 48 +/- 8 ms and T(2) = 6 +/- 2 ms for EC water (15%). Volume-localized ADC(z) (z-gradient axis) values were 0.90 +/- 0.02 (EC+IC), 0.81 +/- 0.05 (IC), 0.51 +/- 0.02 (EC+IC), and 0.53 +/- 0.07 (IC), for normal, CR-infused, ischemic, and ischemic/CR-infused groups, respectively (ADC values are x10(-3) mm(2)/s; n = 5 for each group). Imaging ADC(z) values were 0.81 +/- 0.03 (EC+IC), 0.75 +/- 0.05 (IC), 0.51 +/- 0.04 (EC+IC), and 0.52 +/- 0.05 (IC), respectively, for the same groups. Imaging ADC(av) (average diffusivity) values for the same groups were 0.70 +/- 0.05 (EC+IC), 0.69 +/- 0.06 (IC), 0.45 +/- 0.06 (EC+IC), and 0.44 +/- 0.06 (IC), respectively. These results suggest that the IC water ADC determines the overall water ADC value in normal and ischemic rat brain.
DOI of Published Version
10.1002/mrm.10296
Source
Magn Reson Med. 2002 Nov;48(5):826-37. Link to article on publisher's site
Journal/Book/Conference Title
Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine
Related Resources
PubMed ID
12417997
Repository Citation
Silva MD, Omae T, Helmer KG, Li F, Fisher M, Sotak CH. (2002). Separating changes in the intra- and extracellular water apparent diffusion coefficient following focal cerebral ischemia in the rat brain. Neurology Publications. https://doi.org/10.1002/mrm.10296. Retrieved from https://escholarship.umassmed.edu/neuro_pp/95