Differences in ischemic lesion evolution in different rat strains using diffusion and perfusion imaging
Department of Neurology
Animals; Brain Ischemia; Cerebrovascular Circulation; Coloring Agents; Diffusion; Diffusion Magnetic Resonance Imaging; Disease Models, Animal; Infarction, Middle Cerebral Artery; Ischemia; Magnetic Resonance Angiography; Male; Rats; Rats, Inbred WKY; Rats, Sprague-Dawley; Species Specificity; Tetrazolium Salts; Time Factors
Neurology | Neuroscience and Neurobiology
BACKGROUND AND PURPOSE: Interstrain differences in the temporal evolution of ischemia after middle cerebral artery occlusion (MCAO) in rats may considerably influence the results of experimental stroke research. We investigated, in 2 commonly used rat strains (Sprague-Dawley [SD] and Wistar-Kyoto [WK]), the spatiotemporal evolution of ischemia after permanent suture MCAO using diffusion and perfusion imaging.
METHODS: Serial measurements of quantitative cerebral blood flow (CBF) and apparent diffusion coefficient (ADC) were performed up to 210 min after MCAO. Lesion volumes were calculated by using previously established viability thresholds and correlated with infarct volume defined by 2,3,5-triphenyltetrazolium chloride staining 24 hours after MCAO.
RESULTS: While the ADC-derived lesion volume increased rapidly during the first 120 min after MCAO and essentially stopped growing after 3 hours in SD rats, ADC lesion in WK rats increased progressively during the entire 210-min period and was significantly smaller at all time points (PCONCLUSIONS: This study demonstrated substantial differences in acute ischemic lesion evolution between SD and WK rats. These interstrain variations must be taken into account when assessing new therapeutic approaches on ischemic lesion evolution in the rat MCAO model.
DOI of Published Version
Stroke. 2005 Sep;36(9):2000-5. Epub 2005 Jul 21. Link to article on publisher's site
Stroke; a journal of cerebral circulation
Bardutzky, Juergen F.; Shen, Qiang; Henninger, Nils; Bouley, James P.; Duong, Timothy Q.; and Fisher, Marc, "Differences in ischemic lesion evolution in different rat strains using diffusion and perfusion imaging" (2005). Neurology Publications and Presentations. 61.